Τίτλος:
Evaluation of double immunocytochemical staining for CK20 and P53 as a
potential adjunct to cytology for urothelial cancer diagnosis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objective: To evaluate double immunocytochemical staining with p53 and
CK20, as a tool for improving the accuracy of urinary cytology. The aim
of the present study was to clarify the diagnostic significance of the
expression of these markers and to investigate the possibility of using
this information for better monitoring of bladder cancer patients during
follow-up.
Material and methods: One hundred and twenty-five urine cytology cases
were retrieved with corresponding histology from our files. One ThinPrep
(R) smear was available for each of them and dual immunocytological
staining for p53 and CK20 was performed. Eleven cases were excluded from
the study because of hypocellularity. The material comprised 58
malignant, 36 atypical and 20 negative for malignancy cases.
Immunocytochemistry was evaluated by two cytopathologists, blinded to
the histological diagnosis or follow-up data. A cut-off threshold of
five stained atypical cells, according to the literature, was used for
evaluation.
Results: Fifty-two out of 58 malignant cases were positive for at least
one of the markers (89.6%). In the atypical and negative groups, 18
(50%) and 5 (25%) cases were positive, respectively. Accuracy
parameters evaluation for cytology versus the combination of cytology
with immunocytological staining were: sensitivity 73.4% versus 91.1%,
specificity 100% versus 74.3%, positive predictive value (PPV) 100%
versus 88.9% and negative predictive value (NPV) 62.5% versus 78.8%.
Conclusions: Double immunocytochemical staining for p53 and CK20 is easy
to perform and evaluate and can improve cytology sensitivity. It is
helpful in establishing a diagnosis of malignancy and may be used as a
triage tool to select patients that require cystoscopy during clinical
follow-up.
Συγγραφείς:
Lenos, M.
Katafigiotis, I.
Vrettou, K.
Papaioannou, D. and
Malta, F.
Trigka, E. -A.
Sousouris, S.
Constantinides, C.
and Mikou, P.
Λέξεις-κλειδιά:
urine; cytology; p53; CK20
