Περίληψη:
Background: Cytokine interleukin (IL) 31 has emerged as an important
component of allergic and inflammatory diseases associated with
pruritus, such as atopic dermatitis (AD) and mastocytosis. Mast cells
(MC) are stimulated by allergic and nonallergic triggers, and play a
critical role in such diseases by secreting histamine and tryptase as
well as cytokines and chemokines. IL-33 has been reported to augment MC
responses, but its effect on secretion of IL-31 is not known.
Objectives: To investigate whether IL-33 can stimulate the secretion of
IL-31 from cultured human MCs and whether this response is augmented by
either the neuropeptide substance P (SP) or immunoglobulin E (IgE) and
anti-IgE in the absence or presence of IL-4.
Methods: Laboratory of Allergic Diseases (LAD2) human MCs were cultured
in StemProH-34 SFM medium supplemented by stem cell factor and were
stimulated either with IL-33 (10 ng /mL) or SP (2 mu M), or preincubated
with IgE (1 mu g/mL) overnight, and then stimulated with anti-IgE (1 mu
g/mL) for 24 hours. IL-31 gene expression was measured by quantitative
polymerase chain reaction, and protein was measured by enzyme-linked
immunosorbent assay.
Results: IL-33 (10 ng/mL) induces IL-31 gene expression, synthesis, and
secretion from LAD2 cells in the absence of degranulation, whereas SP
and IgE on their own have no effect. However, the effect of IL-33 is
augmented by SP (2 mu M) and/or IgE and anti-IgE (1 mu g/mL both) and
especially their combination. Moreover, this response is significantly
further increased when LAD2 cells are cultured in the presence of IL-4.
Conclusion: These findings provide evidence that IL-33 induced secretion
of IL-31 from LAD2 MC, an action augmented by novel neuroimmune
interactions that may help in the development of new treatments of
allergic and inflammatory diseases, especially AD and mastocytosis.
Συγγραφείς:
Petra, Anastasia I.
Tsilioni, Irene
Taracanova, Alexandra and
Katsarou-Katsari, Alexandra
Theoharides, Theoharis C.
