Τίτλος:
A Role for Circular Non-Coding RNAs in the Pathogenesis of Sporadic
Parathyroid Adenomas and the Impact of Gender-Specific Epigenetic
Regulation
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Epigenetic changes, including altered small non-coding RNAs, appear to
be implicated in the pathogenesis of sporadic parathyroid adenomas
(PAs). In this study, we investigated the circular RNAs (circRNAs)
expression profile in sporadic PAs. Sixteen tissue samples of sporadic
PAs, and four samples of normal parathyroid tissue (NPT) were
investigated. Sample preparation and microarray hybridization were
performed based on the Arraystar's standard protocols, and circRNAs
sequences were predicted by bioinformatics tools. We identified 35
circRNAs that were differentially expressed in sporadic PAs compared to
NPT; 22 were upregulated, and 13 were downregulated, according to the
pre-defined thresholds of fold-change > 2.0 and p < 0.05. In the
subgroup analysis of PAs from male patients (n = 7) compared to PAs from
female patients (n = 9), we also find a different expression profile. In
particular, 19 circRNAs were significantly upregulated, and four
circRNAs were significantly downregulated in male patients, compared to
female counterparts. We show here for the first time a differential
circRNA expression pattern in sporadic PAs compared to NPT, and a
different expression profile in PA samples from male compared to female
patients, suggesting an epigenetic role in the PA pathogenesis, and also
an effect of gender in the epigenetic regulation of PAs.
Συγγραφείς:
Yavropoulou, Maria P.
Poulios, Christos
Michalopoulos, Nickos
and Gatzou, Ariadni
Chrisafi, Sofia
Mantalovas, Stylianos and
Papavramidis, Theodosis
Daskalaki, Emily
Sofou, Electra and
Kotsa, Kalliopi
Kesisoglou, Isaak
Zebekakis, Pantelis
Yovos,
John G.
Περιοδικό:
Cell Stem Cell
Λέξεις-κλειδιά:
parathyroid adenomas; pathogenesis; epigenetics; non-coding RNAs;
circular RNAs
DOI:
10.3390/cells8010015
