Ερευνητικό υλικό ΕΚΠΑ

The concordance of the limiting antigen and the Bio-Rad avidity assays
in persons from Estonia infected mainly with HIV-1 CRF06_cpx

Αγγλικά

Background
Serological assays to determine HIV incidence have contributed to
estimates of HIV incidence, monitoring of HIV spread, and evaluation of
prevention strategies. Two frequently used incidence assays are the
Sedia HIV-1 LAg-Avidity EIA (LAg) and the Bio-Rad avidity incidence
(BRAI) assays with a mean duration of recent infection (MDRI) of 130 and
240 days for subtype B infections, respectively. Little is known about
how these assays perform with recombinant HIV-1 strains. We evaluated
the concordance of these assays in a population infected mainly with
HIV-1 CRF06_cpx.
Material/Methods
Remnant serum samples (n = 288) collected from confirmed,
newly-diagnosed HIV-positive persons from Estonia in 2013 were tested.
Demographic and clinical data were extracted from clinical databases.
LAg was performed according to the manufacturer’s protocol and BRAI
testing was done using a validated protocol. Samples with LAg-pending or
BRAI-invalid results were reclassified as recent if they were from
persons with viral loads <1000 copies/mL or were reclassified as
long-term if presenting with AIDS.
Results
In total 325 new HIV infections were diagnosed in 2013 in Estonia. Of
those 276 persons were tested with both LAg and BRAI. Using assay
results only, the recency rate was 44% and 70% by LAg and BRAI,
respectively. The majority of samples (92%) recent by LAg were recent
by BRAI. Similarly, 89% of samples long-term by BRAI were long-term by
LAg. After clinical information was included in the analysis, the
recency rate was 44% and 62% for LAg and BRAI, respectively. The
majority of samples (86%) recent by LAg were recent by BRAI and 91% of
long-term infections by BRAI were long-term by LAg.
Conclusions
Comparison of LAg and BRAI results in this mostly CRF06_cpx-infected
population showed good concordance for incidence classification. Our
finding of a higher recency rate with BRAI in this population is likely
related to the longer MDRI for this assay.

2019

Huik, Kristi
Soodla, Pilleriin
Pauskar, Merit
Owen, S.
Michele
Luo, Wei
Murphy, Gary
Jogeda, Ene-Ly
Kallas,
Eveli
Rajasaar, Heli
Avi, Radko
Masciotra, Silvina and
Lutsar, Irja
Del Amo, Julia
Meyer, Laurence
Bucher, Heiner
C.
Chene, Genevieve
Hamouda, Osamah
Pillay, Deenan and
Prins, Maria
Rosinska, Magda
Sabin, Caroline
Touloumi, Giota
and Porter, Kholoud
Olson, Ashley
Cartier, Andrea
Fradette,
Lorraine
Walker, Sarah
Babiker, Abdel
De Luca, Andrea and
Fisher, Martin
Muga, Roberto
Kelleher, Tony
Cooper, David
and Grey, Pat
Finlayson, Robert
Bloch, Mark
Ramacciotti, Tim
and Gelgor, Linda
Smith, Don
Zangerle, Robert
Gill, John and
Dabis, Francois
Thiebaut, Rodolphe
Costagliola, Dominique and
Guiguet, Marguerite
Vanhems, Philippe
Chaix, Marie-Laure and
Ghosn, Jade
Boufassa, Faroudy
Meixenberger, Karolin
Bannert,
Norbert
Bartmeyer, Barbara
Antoniadou, Anastasia
Chrysos,
Georgios
Daikos, Georgios L.
Pantazis, Nikos
Katsarou, Olga
and Rezza, Giovanni
Dorrucci, Maria
Monforte, Antonella
d'Arminio
Geskus, Ronald
van der Helm, Jannie
Schuitemaker,
Hanneke
Sannes, Mette
Dyrhol-Riise, Anne Ma
Kran, Anne-Marte
Bakken
Rosinska, Magdalena
Tor, Jordi
de Olalla, Patricia
Garcia
Cayla, Joan
del Amo, Julia
Moreno, Santiago and
Monge, Susana
del Romero, Jorge
Perez-Hoyos, Santiago and
Sonnerborg, Anders
Guenthard, Huldrych
Scherrer, Alexandra and
Malyuta, Ruslan
Johnson, Anne
Phillips, Andrew
Morrison,
Charles
Salata, Robert
Mugerwa, Roy
Chipato, Tsungai and
Price, Matt A.
Gilmour, Jill
Kamali, Anatoli
Karita, Etienne
and Burns, Fiona
Giaquinto, Carlo
Grarup, Jesper
Kirk, Ole
and Bailey, Heather
Anne, Alain Volny
Panteleev, Alex and
Thorne, Claire
Aboulker, Jean-Pierre
Albert, Jan
Asandi,
Silvia
De Wit, Stephane
Reiss, Peter
Gatell, Jose and
Karpov, Igor
Ledergerber, Bruno
Lundgren, Jens
Moller, Claus
and Rakhma-nova, Aza
Rockstroh, Juergen
Sandhu, Manjinder and
Dedes, Nikos
Fenton, Kevin
Pizzuti, David
Vitoria, Marco and
Faggion, Silvia
Frost, Richard
Raben, Dorthe
Schwimmer,
Christine
Scott, Martin
CASCADE Collaboration EuroCoord

PLOS ONE

Public Library of Science

14

5

https://doi.org/10.1371/journal.pone.0217048

10.1371/journal.pone.0217048

https://pergamos.lib.uoa.gr/uoa/dl/object/3183965