Περίληψη:
With new or emerging fungal infections, human and animal fungal
pathogens are a growing threat worldwide. Current diagnostic tools are
slow, non-specific at the species and subspecies levels, and require
specific morphological expertise to accurately identify pathogens from
pure cultures. DNA barcodes are easily amplified, universal, short
species-specific DNA sequences, which enable rapid identification by
comparison with a well-curated reference sequence collection. The
primary fungal DNA barcode, ITS region, was introduced in 2012 and is
now routinely used in diagnostic laboratories. However, the ITS region
only accurately identifies around 75% of all medically relevant fungal
species, which has prompted the development of a secondary barcode to
increase the resolution power and suitability of DNA barcoding for
fungal disease diagnostics. The translational elongation factor 1 alpha
(TEF1 alpha) was selected in 2015 as a secondary fungal DNA barcode, but
it has not been implemented into practice, due to the absence of a
reference database. Here, we have established a quality-controlled
reference database for the secondary barcode that together with the
ISHAM-ITS database, forms the ISHAM barcode database, available online
at http://its.mycologylab.org/. We encourage the mycology community for
active contributions.
Συγγραφείς:
Meyer, Wieland
Irinyi, Laszlo
Minh Thuy Vi Hoang
Robert,
Vincent
Garcia-Hermoso, Dea
Desnos-Ollivier, Marie
Yurayart,
Chompoonek
Tsang, Chi-Ching
Lee, Chun-Yi
Woo, Patrick C. Y.
and Pchelin, Ivan Mikhailovich
Uhrlass, Silke
Nenoff, Pietro and
Chindamporn, Ariya
Chen, Sharon
Hebert, Paul D. N.
Sorrell,
Tania C.
Halliday, Catriona
Arthur, Ian
Moretti, Maria Luiza
and de Almeida Soares, Celia Maria
Muniz, Mauro de Medeiros and
Zancope-Oliveira, Rosely Maria
Cruz, Fundacao Oswaldo
Melo,
Analy Salles de Azevedo
Colombo, Arnaldo L.
Nishikaku, Angela
Satie
Hendrickx, Marijke
Stubbe, Dirk
Normand, Anne-Cecile
and Piarroux, Renaud
Ranque, Stephane
Dromer, Francoise and
Arabatzis, Michael
Velegraki, Aristea
Cardinali, Gianluigi and
Castanon, Laura Rosio
Taylor, Maria Lucia
Toriello, Conchita and
de Hoog, Sybren
Pais, Celia
de Beer, Wilhelm
Gryzenhout,
Marieka
Guarro, Josep
Cano-Lira, Jose F.
Robbertse, Barbara
and Schoch, Conrad
ISHAM Barcoding Pathogenic Fungi
