Τίτλος:
Platelet-rich Plasma and Mesenchymal Stem Cells Local Infiltration
Promote Functional Recovery and Histological Repair of Experimentally
Transected Sciatic Nerves in Rats
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Introduction
Platelet-rich plasma (PRP) products and mesenchymal stem cells (MSCs)
seem to have a significant potential as neurogenic therapeutic modulator
systems. This study aimed to investigate such biological blood
derivatives that could enhance nerve regeneration when applied locally
in the primary repair of peripheral nerve transection of an experimental
rat model.
Methods
A total of 42 two-month-old male Wistar rats were divided into three
“treatment” groups (control, PRP, and MSCs). All the subjects were
operated under anesthesia, and the surgical site was infiltrated with
either normal saline, PRP derived from the animal’s peripheral blood, or
MSCs derived from the animal’s femoral bone marrow. All three groups
were also sub-divided into two sub-groups based on the post-operative
administration of Non-steroidal anti-inflammatory drugs (NSAIDs) or not
in order to evaluate the effect of NSAIDs on the final outcome. Three
months post-surgery, electromyography evaluation of both hind limbs
(right operated and left non-operated) was performed. The animals were
euthanized, and nerve repair specimens were prepared for histology.
Results
PRP group had a significant effect (p<0.05) on the sciatic nerve repair
when compared with the control group, whereas the MSC group had a
positive effect but was not statistically significant (p=0.2). The
number of counted neural axons at the area distal to the nerve repair
site were significantly repetitive (p<0.05) in both the PRP and MSC
groups when compared with the control group.
Conclusions
Both PRP and MSCs appear to play an essential role in the enhancement of
nerve repair in terms of functionality and histology. MSCs group
demonstrated a positive effect, whereas the PRP group showed
statistically significant better results.
Συγγραφείς:
Kokkalas, Nikolaos
Kokotis, Panagiotis
Diamantopoulou, Kalliopi
and Galanos, Antonios
Lelovas, Pavlos
Papachristou, Dionysios J.
and Dontas, Ismene A.
Triantafyllopoulos, Ioannis K.
Λέξεις-κλειδιά:
plateler rich plasma; mesenchymal stem cells; nerve repair; nerve
regeneration
