Τίτλος:
Next-generation sequencing reveals alternative L -DOPA decarboxylase
(DDC) splice variants bearing novel exons, in human hepatocellular and
lung cancer cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The human L-DOPA decarboxylase (DDC) is an enzyme that displays a
pivotal role in metabolic processes. It is implicated in various human
disorders, including hepatocellular and lung cancer. Several splice
variants of DDC have previously been described, most of which encode for
protein isoforms of this enzyme. In the present study, we used
next-generation sequencing (NGS) technology along with nested touchdown
PCR and Sanger sequencing to identify new splice variants bearing novel
exons of the DDC gene, in hepatocellular and lung cancer cell lines.
Using an in-house-developed algorithm, we discovered seven novel DDC
exons. Next, we determined the structure of ten novel DDC transcripts,
three of which contain an open reading frame (ORF) and probably encode
for three previously unknown protein isoforms of this enzyme. Future
studies should focus on the elucidation of their role in cellular
physiology and cancer pathobiology.
Συγγραφείς:
Papatsirou, Maria
Adamopoulos, Panagiotis G.
Artemaki, I,
Pinelopi
Georganti, Vasiliki P.
Scorilas, Andreas and
Vassilacopoulou, Dido
Kontos, Christos K.
Περιοδικό:
Genes and Nutrition
Λέξεις-κλειδιά:
Aromatic L-amino acid decarboxylase (AADC); Alternative splicing; Liver
carcinoma; Lung adenocarcinoma; Transcriptional analysis;
High-throughput sequencing
DOI:
10.1016/j.gene.2020.145262
