Περίληψη:
We investigated the expression profile of selected microRNAs (miRs) in
serum and tissue samples from patients with sporadic parathyroid
adenomas (sPAs). This was a prospective, controlled cohort study. Forty
patients with sPAs who had undergone parathyroidectomy (PTX) were
included. MiR extraction was performed from (i) 40 formalin-fixed
paraffin-embedded samples (FFPEs) of sPAs, (ii) 10 FFPEs of normal
parathyroid tissue (NPT), (iii) serum samples of the 40 patients with
sPAs (t1 = baseline; t2 = 2months post-PTX), and (vi) serum samples of
10 healthy individuals (controls; t1 = baseline and t2 = 2months later).
Ten miRs were selected based on their interaction with genes related to
parathyroid tumorigenesis (miR-17-5p, miR-24-3p, miR-29b-3p, miR-31-5p,
miR-135b-5p, miR-186-5p, miR-195-5p, miR-330-3p, miR-483-3p, and
miR-877-5p). At tissue level, the relative expression of miR-17-5p,
miR-31-5p, miR-135b-5p, miR-186-5p, and miR-330-3p was significantly
decreased (fold change [FC]: 0.17, FC: 0.03, FC: 0.01, FC: 0.10, FC:
0.10, respectively; all p values <0.001), and the expression of
miR-24-3p and miR-29b-3p was significantly increased (FC: 12.4, p<0.001;
FC: 18.5, p = 0.011, respectively) in sPA compared with NPT samples. The
relative expression of miR-135b-5p was also significantly decreased in
the serum samples of patients compared with controls (FC: 0.7, p =
0.035). No significant differences were found in the serum samples of
patients before and after PTX. MiRs that regulate genes linked to
parathyroid tumors such as menin 1 (miR-24-3p, miR-29b-3p), cyclin D1
(miR-17-5p), calcium sensing receptor (miR-31-5p, miR-135b-5p),
cyclin-dependent kinase inhibitors (miR-186-5p), and beta -catenin
(miR-330-3p) were significantly deregulated in sPAs compared with NPT
samples, suggesting a role for epigenetic changes in parathyroid
tumorigenesis. (c) 2020 The Authors. JBMR Plus published by Wiley
Periodicals LLC on behalf of American Society for Bone and Mineral
Research.
Συγγραφείς:
Yavropoulou, Maria P.
Pazaitou-Panayiotou, Kalliopi
Yovos, John
G.
Poulios, Christos
Anastasilakis, Athanasios D. and
Vlachodimitropoulos, Dimitris
Vambakidis, Kyriakos
Tsave, Olga
and Chrisafi, Sofia
Daskalaki, Emily
Makras, Polyzois
