Περίληψη:
The present study investigates the impacts of low pH on the cell structure of the seagrasses Posidonia oceanica (L.) Delile and Cymodocea nodosa (Ucria) Ascherson. The study was conducted via in situ experiments performed at the Castello Aragonese of Ischia (Naples, Italy), where shallow submarine vents lower the pH and can be used as natural laboratories. Shoots of the seagrasses were transferred from the control area (pH 8.1) to the two venting areas (pH 7.8 and 6.8) at different times. Epidermal cells of young leaves were examined using transmission electron microscopy (TEM) and tubulin immunofluorescence. After one week at pH 7.8, the cell structure of Posidonia oceanica was normal, while in Cymodocea nodosa, microtubule (MT) network and cell structure were affected. In addition, in C. nodosa, ultrastructural analysis revealed a gradual degradation of the nuclei, a disorganization of the chloroplasts, and an increase in the number of mitochondria and dictyosomes. The exposure of both plants for 3 weeks at pH 6.8 resulted in the aggregation and finally in the dilation of the endoplasmic reticulum (ER) membranes. Tubulin immunofluorescence revealed that after three weeks, the MT cytoskeleton of both plants was severely affected. All these alterations can be considered as indications of an apoptotic-like programmed cell death (AL-PCD), which may be executed in order to regulate stress response. © 2022
Συγγραφείς:
Koutalianou, M.
Buia, M.C.
Katsaros, C.
