Τίτλος:
The effect of hepatocyte growth factor on intestinal adaption in an experimental model of short bowel syndrome
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Purpose: Nowadays, the standard therapy for patients with short bowel syndrome is parenteral nutrition (PN). Various growth factors have been tested to achieve weaning from prolonged PN administration. We evaluated the effect of hepatocyte growth factor (HGF) on structural intestinal adaptation and cell proliferation in a rat model of SBS. Methods: Thirty Sprague–Dawley rats were divided into three groups; group A rats (sham) underwent bowel transection, group B rats underwent a 75% bowel resection, and group C rats underwent the same procedure but were treated postoperatively with HGF. Histopathologic parameters of intestinal adaptation were determined, while microarray and rt-PCR analyses of ileal RNA were also performed. Results: Treatment with HGF resulted in significant increase in body weight, while the jejunal and ileal villus height and crypt depth were increased in HGF rats (36%, p < 0.05 and 27%, p < 0.05 respectively). Enterocyte proliferation was also significantly increased in HGF rats (21% p < 0.05). Microarray and quantitative rt-PCR analyses showed that the genes hgfac, rac 1, cdc42, and akt 1 were more than twofold up-regulated after HGF treatment. Conclusion: HGF emerges as a growth factor that enhances intestinal adaptation. The future use of HGF may potentially reduce the requirement for PN in SBS patients. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Συγγραφείς:
Bagias, G.
Misiakos, E.P.
Charalampopoulos, A.
Zavras, N.
Sakellariou, S.
Schizas, D.
Sukhotnik, I.
Giamarelos, E.
Pikoulis, E.
Περιοδικό:
Pediatric Surgery International
Εκδότης:
Springer Science and Business Media Deutschland GmbH
Λέξεις-κλειδιά:
glyceraldehyde 3 phosphate dehydrogenase; growth factor; hepatocyte growth factor activator; ketamine; protein Cdc42; protein kinase B; Rac1 protein; scatter factor; unclassified drug; xylazine; scatter factor, anastomosis; animal cell; animal experiment; animal model; animal tissue; apoptosis rate; Article; body weight; body weight gain; cell proliferation; controlled study; drug effect; female; food intake; general anesthesia; general condition; histopathology; housekeeping gene; ileocecal valve; ileus; immunohistochemistry; intestinal adaptation; intestine cell; intestine crypt; intestine function; intestine resection; intestine villus; jejunoileostomy; jejunum; microarray analysis; morphometry; nonhuman; optimal drug dose; parenteral nutrition; pneumothorax; rat; real time polymerase chain reaction; short bowel syndrome; upregulation; adaptation; animal; disease model; genetics; intestine; intestine mucosa; metabolism; pathology; short bowel syndrome; Sprague Dawley rat; theoretical model, Adaptation, Physiological; Animals; Disease Models, Animal; Hepatocyte Growth Factor; Intestinal Mucosa; Intestines; Models, Theoretical; Rats; Rats, Sprague-Dawley; Short Bowel Syndrome
DOI:
10.1007/s00383-022-05341-6
