Κατεύθυνση Απομόνωση, Ανάπτυξη, Παραγωγή και Έλεγχος Βιοδραστικών Φυσικών Προϊόντων
Library of the School of Science

2023-05-19

2023

Papalexis Patroklos

Νεκτάριος Αληγιάννης, Αναπληρωτής Καθηγητής Τμήμα Φαρμακευτικής ΕΚΠΑ,
Νικόλαος Φωκιαλάκης Επίκ. Καθηγητής Τμήμα Φαρμακευτικής ΕΚΠΑ,
Δρ. Αικ. Τερμεντζή (Ερευνήτρια Β΄ - Μπενάκειο Φυτοπαθολογικό Ινστιτούτο

Μελέτη μεταβολικού προφίλ ολικών εκχυλισμάτων φύλλων και καρπών του είδους Pistacia lentiscus με χρήση 1H-NMR και συσχέτιση της δράσης τους ως αντιμικροβιακοί παράγοντες και αναστολείς του ενζύμου της τυροσινάσης

Greek
English

1H-NMR metabolic profiling of Pistacia lentiscus leaves and fruit total extracts for the identification of biomarkers associated with antimicrobial properties and tyrosinase enzyme inhibitory properties.

Pistacia lentiscus L. is an evergreen shrub that grows in the Mediterranean region at
an altitude of up to 1000 m above sea level. The family Anacardiaceae, to which the
species belongs, is part of the wider category of Angiosperms and includes flowering
plants, trees or shrubs with small and usually indistinguishable flowers. The plants of
this family exhibit milky or resinous secretions which are aromatic but at the same
time can be irritating to the skin.
Pistacia lentiscus L. produces a wide variety of secondary metabolites. The main
categories that stand out in the species are a) phenylpropanoids such as flavonoids,
tannins, phenolics and their derivatives, b) terpenoids and in particular
monoterpenes, sesquiterpenes, diterpenes, triterpenic acids and their derivatives,
and d) lipids e) carotenoids.
Plants of the genus P. lentiscus have been extensively studied and have shown many
interesting pharmacological actions / effects. The main ones, which concern the
aboveground parts of the plant, the leaves and the fruits, seem to be: antioxidants,
antimicrobials, anti-inflammatory, hepatoprotective as well as the inhibition of
mutagenic agents.
In the present study we focused on capturing a snapshot of the metabolic profile of
total leaf and fruit extracts of the species, which are considered by-products in the
Chios mastic (resin) production industry. Our aim was to study the differences in
secondary metabolites between fruit and leaf samples but also the similarities or
differences in the metabolic profile of samples from the same parts of the plant from
different regions. The aim was to investigate the utilization of the leaves and fruits of
the mastic tree, which are considered by-products, and their study as a source of
bioactive secondary metabolites.
Briefly, the total methanolic extracts of the leaf samples were tested for their activity
as antimicrobial agents and for their activity as tyrosinase inhibitors. At the same time,
the total methanolic extracts of the fruit samples were evaluated for their action as
tyrosinase inhibitors. The aim was to test the activity of the total extracts and to study
the covariance of their biological activity with the spectral information obtained from
the NMR spectra using multivariate statistical analysis software.
The ultimate goal is to optimize plant species study methods, reducing the time and
cost required by conventional studies to find new bioactive metabolites, bypassing the
stages of fractionation and isolation of metabolites.

Science

Health Sciences

Pistacia, lentiscus, metabolites, pharmacological, metabolic, profile, Chios, mastic, methanolic, extracts, antimicrobial, agents, tyrosinase, inhibitors, NMR, spectra, multivariate, statistics

Yes

3

Yes

116

179

File access is restricted until 2026-05-26.

https://pergamos.lib.uoa.gr/uoa/dl/object/3328697