Τίτλος:
In vitro response of hFOB cells to pamidronate modified sodium silicate coated cellulose scaffolds
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The aim of the present study was to evaluate the suitability of cellulose-based scaffolds coated with pure sodium silicate gel and sodium silicate gels accumulated with different concentrations of the bisphosphonate pamidronate as scaffolds for attachment, proliferation and differentiation of human fetal osteoblasts (hFOB 1.19). Human osteoblasts were cultured in vitro for a period up to 14 days on different cellulose scaffolds. Unmodified and sodium silicate coated cellulose scaffolds were used as control. Two surface-coated modifications of cellulose were applied. The scaffolds were coated in a modified two-step dip coating process with pure sodium silicate gel and pamidronate enriched sodium silicate gel, respectively. In order to investigate the influence of the pamidronate, concentrations of 0.667 mg Na-pamidronate/ml sodium silicate solution, 0.333 mg Na-pamidronate/ml sodium silicate solution and 3.33 × 10-3 mg Na-pamidronate/ml sodium silicate solution were used for the coating process. Cell proliferation, vitality and attachment were examined by means of cell counting, WST-1 test, fluorescence and scanning electron microscopy. The relative grade of differentiation of hFOB cells was examined by using quantitative real-time polymerase chain reaction (qRT-PCR) analysis for the gene expression of alkaline phosphatase and osteocalcin. Proliferation and differentiation of human osteoblasts was enhanced by the sodium silicate coatings accumulated with pamidronate compared to pure sodium silicate coatings. There was a reciprocal correlation of vitality with the concentration of pamidronate. The highest vitality was found on surfaces with the lowest pamidronate accumulation. Alkaline phosphatase, an early differentiation marker, was overexpressed after 7 days in cells on all pamidronate-containing surfaces (up to 350% compared to untreated cellulose). Osteocalcin, a late differentiation marker, was overexpressed after 14 days in cells on all coated surfaces (up to 300,000% compared to untreated cellulose). The results indicate that due to the modified coating procedure a homogeneous coating and thus, an enhancement of cell attachment and subsequent cellular functions can be achieved. Low concentrations of pamidronate seem to have a relevant effect on cell proliferation and vitality and, therefore, can be recommended for the improvement of the properties of a biomaterial. © 2008 Elsevier B.V. All rights reserved.
Συγγραφείς:
Ponader, S.
Brandt, H.
Vairaktaris, E.
von Wilmowsky, C.
Nkenke, E.
Schlegel, K.A.
Neukam, F.W.
Holst, S.
Müller, F.A.
Greil, P.
Περιοδικό:
Colloids and Surfaces B: Biointerfaces
Λέξεις-κλειδιά:
Cellulose; Chemical modification; Fluorescence; Osteoblasts; Scanning electron microscopy; Sodium compounds, Bisphosphonates; Human osteoblasts; In vitro cell response; Sol-gel coating, Silicates, alkaline phosphatase; cellulose; molecular scaffold; osteocalcin; pamidronic acid; silicate; silicate sodium, article; cell adhesion; cell differentiation; cell proliferation; controlled study; gene expression; human; human cell; in vitro study; osteoblast; priority journal; real time polymerase chain reaction; scanning electron microscopy, Alkaline Phosphatase; Biological Markers; Cell Adhesion; Cell Differentiation; Cell Proliferation; Cell Survival; Cells, Cultured; Cellulose; Coated Materials, Biocompatible; Diphosphonates; Dose-Response Relationship, Drug; Fetus; Gene Expression; Humans; Osteoblasts; Osteocalcin; Silicates; Time Factors; Tissue Scaffolds, Cellulose; Sodium Silicate
DOI:
10.1016/j.colsurfb.2008.02.002
