Τίτλος:
Improvement of Lysine Production by Analog-Sensitive and Auxotroph Mutants of the Acetylene-Utilizing Bacterium Gordona bronchialis (Rhodococcus bronchialis)
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
An acetylene utilizing Gordona (Rhodococcus) bronchialis strain, screened for the production of fine chemicals, was found to be capable of producing small amounts of lysine. Attempts to produce amino-acid analog-resistant and/or sensitive mutants and auxotrophs of this strain with increased lysine production were made following UV-irradiation or N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) treatment. The bacterium exhibited surprisingly high resistance levels to the aforementioned mutagens which is attributed to highly effective inborn-repair systems. Natural resistance to high levels of S-(2-aminoethyl)-L-cysteine (AEC) (2%) was observed, in contrast with D, L-aspartic acid hydroxamate (AAH), L-lysine hydroxamate (LHX) and β-fluoropyruvate (FP). A variety of amino-acid analog-resistant (AAHr, LHXr) or analog-sensitive (FPs) mutants were produced following UV-irradiation or MNNG treatment. Similarly, a large number of auxotrophs (68) of different types were also obtained. From these, one FPs mono-auxotroph and two poly-auxotrophs (with at least one requirement for the aspartic acid family) showed an increased lysine production (∼1.8 g/L) comparable (4 g/L) to that found in other bacteria capable of utilizing long-chain hydrocarbons (1).
Συγγραφείς:
Kyriacou, A.
Balis, C.
Typas, M.A.
Περιοδικό:
Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Εκδότης:
Humana Press Inc.
Λέξεις-κλειδιά:
Bacteria (microorganisms); Gordonia bronchialis; Rhodococcus, acetylene; asparagine; beta aspartylhydroxamic acid; beta-aspartylhydroxamic acid; cysteine; drug derivative; lysine; lysine hydroxamate; pyruvic acid derivative; s (2 aminoethyl)cysteine; S-2-aminoethyl cysteine, antibiotic resistance; article; biosynthesis; metabolism; mutation; Rhodococcus, Acetylene; Asparagine; Cysteine; Drug Resistance, Microbial; Lysine; Mutation; Pyruvates; Rhodococcus
