Τίτλος:
Effect of L-carnitine administration on the modulated rat brain protein concentration, acetylcholinesterase, Na+K+-ATPase and Mg2+-ATPase activities induced by forced swimming
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Forced exercise produces free radicals and L-carnitine (L-C) administration reduces oxidative stress. Aim: To investigate whether short (2 hours) or prolonged (3 hours) forced swimming could modulate total anti-oxidant status (TAS), protein concentration and activities of acetylcholinesterase (AChE), Na+K+-ATPase and Mg2+-ATPase in rat brain following intraperitonal administration of L-C (300 mg/kg). Methods: TAS, protein and enzyme activities were measured spectrophotomethcally. Results: TAS, protein concentration and AChE activity were reduced, whereas Na +K+-ATPase and Mg2+-ATPase were significantly increased after either 2 or 3 hours of training. L-C administration resulted in a profound restoration of TAS and protein concentration whereas AChE and Na +K+-ATPaSe were increased before exercise, followed by AChE restoration and Na+K+-ATPase reduction after exercise. Mg2+-ATPase remained unchanged. An in vitro study using L-C incubation of brain homogenates previously treated with L-C resulted in complete restoration of the modulated enzymes, whereas the enzyme activities from untreated animals remained unaltered. Conclusions: Short or prolonged swimming in rats may result in a reduction of brain TAS, protein concentration and AChE activity, and an activation of Na+K+-ATPaSe and Mg2+-ATPase. L-C administration may prevent reduction in TAS and protein concentration, and a decrease in AChE and Na+K +-ATPase activity; the latter reached preexercise values after L-C incubation.
Συγγραφείς:
Tsakiris, T.
Angelogianni, P.
Tesseromatis, C.
Tsakiris, S.
Schulpis, K.H.
Περιοδικό:
British Journal of Sports Medicine
Λέξεις-κλειδιά:
acetylcholinesterase; adenosine triphosphatase (magnesium); adenosine triphosphatase (potassium sodium); brain protein; carnitine, animal cell; animal experiment; animal tissue; antioxidant activity; article; brain homogenate; concentration response; controlled study; drug effect; drug research; enzyme activity; enzyme inactivation; exercise; forced swimming test; histology; in vitro study; male; nonhuman; oxidative stress; rat; spectrophotometry; training, Acetylcholinesterase; Animals; Brain; Ca(2+) Mg(2+)-ATPase; Carnitine; Free Radicals; Male; Oxidative Stress; Rats; Rats, Wistar; Sodium-Potassium-Exchanging ATPase; Swimming
DOI:
10.1136/bjsm.2007.039792
