Τίτλος:
Early responses of human pulp to direct capping with resin adhesive systems and calcium hydroxide
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objective: Early responses of human pulp to Prime&Bond/phosphoric acid, Clearfil SE Bond, Clearfil S3 Bond and Dycal were investigated ex vivo. Materials and methods: The three adhesives, Dycal or buffer (DPBS) were applied directly onto the pulp of human teeth slices that were placed in culture for 4 days. Cell viability was monitored by the MTT assay during the culture period. After 4 days, tissue integrity was examined by hematoxylin–eosin staining. Vimentin levels were assessed by Western blotting. TUNEL assay was applied for apoptotic cell detection at specific pulp areas. Results: Profound reduction of cell viability and tissue integrity was observed in adhesive-treated groups, while the impact of Dycal was found to be less harmful. Extended apoptosis was caused mostly by the Clearfil SE and Prime&Bond. All adhesives reduced Vimentin levels. Significance: The study provides evidence that early pulp responses to direct capping with different adhesive systems or calcium hydroxide may vary significantly and underline the need for further studies in relevant ex vivo systems. © 2018 The Academy of Dental Materials
Συγγραφείς:
Poimenova, A.
Kitraki, E.
Kakaboura, A.
Rahiotis, C.
Περιοδικό:
DENTAL MATERIALS
Εκδότης:
ELSEVIER SCIENCE INC 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Λέξεις-κλειδιά:
Adhesives; Calcium; Calcium compounds; Cell death; Hydrated lime; Lime; Pulp; Tissue, Adhesive systems; Apoptotic cells; Direct capping; Dycal; Hematoxylin eosin; Tissue integrity; Vimentin; Western blotting, Pulp materials, biomedical and dental materials; bisphenol A bis(2 hydroxypropyl) ether dimethacrylate; calcium hydroxide; Clearfil S3 Bond; Clearfil SE Bond; dentin bonding agent; Dycal; mineral; polymethacrylic acid derivative; Prime & Bond; resin cement; vimentin, apoptosis; cell survival; cytology; dental pulp capping; drug effect; human; in vitro study; tooth pulp; TUNEL assay; Western blotting, Apoptosis; Bisphenol A-Glycidyl Methacrylate; Blotting, Western; Calcium Hydroxide; Cell Survival; Dental Pulp; Dental Pulp Capping; Dentin-Bonding Agents; Humans; In Situ Nick-End Labeling; In Vitro Techniques; Minerals; Polymethacrylic Acids; Pulp Capping and Pulpectomy Agents; Resin Cements; Vimentin
DOI:
10.1016/j.dental.2018.01.018
