Περίληψη:
It has been recognized that molecular classifications will form the basis for neuropathological diagnostic work in the future. Consequently, in order to reach a diagnosis of Alzheimer's disease (AD), the presence of hyperphosphorylated tau (HP-tau) and β-amyloid protein in brain tissue must be unequivocal. In addition, the stepwise progression of pathology needs to be assessed. This paper deals exclusively with the regional assessment of AD-related HP-tau pathology. The objective was to provide straightforward instructions to aid in the assessment of AD-related immunohistochemically (IHC) detected HP-tau pathology and to test the concordance of assessments made by 25 independent evaluators. The assessment of progression in 7-μm-thick sections was based on assessment of IHC labeled HP-tau immunoreactive neuropil threads (NTs). Our results indicate that good agreement can be reached when the lesions are substantial, i.e., the lesions have reached isocortical structures (stage V-VI absolute agreement 91%), whereas when only mild subtle lesions were present the agreement was poorer (I-II absolute agreement 50%). Thus, in a research setting when the extent of lesions is mild, it is strongly recommended that the assessment of lesions should be carried out by at least two independent observers. © 2008 The Authors.
Συγγραφείς:
Alafuzoff, I.
Arzberger, T.
Al-Sarraj, S.
Bodi, I.
Bogdanovic, N.
Braak, H.
Bugiani, O.
Del-Tredici, K.
Ferrer, I.
Gelpi, E.
Giaccone, G.
Graeber, M.B.
Ince, P.
Kamphorst, W.
King, A.
Korkolopoulou, P.
Kovács, G.G.
Larionov, S.
Meyronet, D.
Monoranu, C.
Parchi, P.
Patsouris, E.
Roggendorf, W.
Seilhean, D.
Tagliavini, F.
Stadelmann, C.
Streichenberger, N.
Thal, D.R.
Wharton, S.B.
Kretzschmar, H.
Λέξεις-κλειδιά:
amyloid beta protein; tau protein, adult; aged; Alzheimer disease; article; brain tissue; clinical article; clinical assessment; clinical laboratory; dementia; disease course; Europe; female; human; human tissue; immunohistochemistry; male; neurofibrillary tangle; neurologic disease; neuropathology; pathologist; protein phosphorylation; senile plaque
