Τίτλος:
Alternations of 14-3-3 θ and β protein levels in brain during experimental sepsis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The 14-3-3 family members play a crucial role in the determination of cell fate, exerting their antiapoptotic activity through directly interfering with the critical function of the mitochondrial core proapoptotic machinery. Dimerization of 14-3-3 is vital for the interaction with many of its client proteins and is regulated by phosphorylation. In a previous study, we observed time-dependent neuronal apoptosis during sepsis. Therefore, in the present study, we sought to evaluate the expression of 14-3-3 θ and β isoforms in septic brain and their association with apoptosis. Sepsis was induced by a CLP model in Wistar rats that were sacrificed at predefined time points. Flow cytometric analysis showed a sepsis-induced, time-dependent alteration of 14-3-3 θ and β isoforms in both Neun + and GFAP + cells. 14-3-3 θ was linearly correlated with apoptosis, and stratified analysis for alive and apoptotic neuronal cells demonstrated a gradual down-regulation of θ isoform in alive neurons and astrocytes. The phospho-P38 (pP38) MAP kinase levels were altered in a time-dependent manner during sepsis, presenting a peak at 6 hr post-CLP. A significant correlation between the two isoforms of 14-3-3 was observed in septic rats, with the θ isoform predominant at all time points. The hippocampus, Purkinje cells, and glia-like cells showed intense immunohistochemical reactivity for 14-3-3 θ isoform, whereas the choroid plexus showed constantly increased β isoform expression. Our results showed that sepsis alters the expression of both 14-3-3 θ and β isoforms in a time-, cell-, and topography-dependent manner. © 2011 Wiley-Liss, Inc.
Συγγραφείς:
Memos, N.
Kataki, A.
Chatziganni, E.
Nikolopoulou, M.
Skoulakis, E.
Consoulas, C.
Zografos, G.
Konstadoulakis, M.
Περιοδικό:
Journal of Neuroscience Research
Λέξεις-κλειδιά:
brain protein; glial fibrillary acidic protein; mitogen activated protein kinase p38; neuron specific nuclear protein; phosphoprotein; protein 14 3 3; protein 14 3 3 beta; protein 14 3 3 theta; unclassified drug, animal cell; animal experiment; animal model; animal tissue; apoptosis; article; astrocyte; brain nerve cell; controlled study; down regulation; experimental infection; hippocampus; immunohistochemistry; male; nerve cell necrosis; nonhuman; priority journal; protein expression; protein localization; Purkinje cell; rat; sepsis; time, 14-3-3 Proteins; Animals; Apoptosis; Brain; Cecum; Disease Models, Animal; Male; Neurons; Protein Isoforms; Rats; Rats, Wistar; Sepsis; Time Factors; Tissue Distribution
