Τίτλος:
Real-life evaluation of a COVID-19 rapid antigen detection test in hospitalized children
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Rapid antigen detection (RAD) tests for the detection of SARS-CoV-2 are simpler, faster, and less expensive than the reverse-transcription polymerase chain reaction (RT-PCR) that is currently considered the gold standard for the diagnosis of coronavirus disease 2019 (COVID-19). The objective of this study was to determine the performance of the PANBIO COVID-19 Ag RAD (Abbott) test, a lateral flow immunoassay that detects the nucleocapsid protein, using as a reference RT-PCR method the Cobas®8800 System (Roche Diagnostics). This prospective study was conducted in a tertiary Children's Hospital and included individuals aged ≤16 years with COVID-19-related symptoms or epidemiological criteria for COVID-19. Two nasopharyngeal samples were collected to perform the PANBIO RAD test and RT-PCR. Of 744 children included, 51 (6.86%) had a positive RT-PCR result. The RAD test detected 42 of 51 PCR-positive children while there were no false-positive results. The overall sensitivity and specificity were 82.35% (95% CI, 71.9%–92.8%) and 100%, respectively. Sensitivity was >95% in symptomatic children. The assay performed poorly in asymptomatically infected children. In agreement with previous studies in adults, the PANBIO RAD test can be useful in screening for COVID-19 in children admitted with symptoms suggestive of the disease, especially in the first days of the illness. © 2021 Wiley Periodicals LLC
Συγγραφείς:
Eleftheriou, I.
Dasoula, F.
Dimopoulou, D.
Lebessi, E.
Serafi, E.
Spyridis, N.
Tsolia, M.
Περιοδικό:
Journal of Medical Virology
Εκδότης:
John Wiley and Sons Inc
Λέξεις-κλειδιά:
coronavirus nucleocapsid protein; virus antigen, adolescent; antigen detection; Article; child; cohort analysis; controlled clinical trial; controlled study; coronavirus disease 2019; diagnostic test accuracy study; female; Greece; hospitalization; human; intermethod comparison; lateral flow immunochromatography; major clinical study; male; nasopharyngeal swab; prospective study; reverse transcription polymerase chain reaction; sensitivity and specificity; symptom; diagnosis; false negative result; false positive result; hospitalization; hospitalized child; immunoassay; infant; isolation and purification; nasopharynx; pandemic; preschool child; procedures; virology, Adolescent; Antigens, Viral; Child; Child, Hospitalized; Child, Preschool; COVID-19; COVID-19 Serological Testing; False Negative Reactions; False Positive Reactions; Female; Hospitalization; Humans; Immunoassay; Infant; Male; Nasopharynx; Pandemics; Prospective Studies; SARS-CoV-2; Sensitivity and Specificity
