Τίτλος:
Genetic features of HIV-1 integrase sub-subtype A6 predominant in Russia and predicted susceptibility to instis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The increasing use of the integrase strand transfer inhibitor (INSTI) class for the treatment of HIV-infection has pointed to the importance of analyzing the features of HIV-1 subtypes for an improved understanding of viral genetic variability in the occurrence of drug resistance (DR). In this study, we have described the prevalence of INSTI DR in a Russian cohort and the genetic features of HIV-1 integrase sub-subtype A6. We included 408 HIV infected patients who were not exposed to INSTI. Drug resistance mutations (DRMs) were detected among 1.3% of ART-naïve patients and among 2.7% of INSTI-naïve patients. The prevalence of 12 polymorphic mutations was significantly different between sub-subtypes A6 and A1. Analysis of the genetic barriers determined two positions in which subtype A (A1 and A6) showed a higher genetic barrier (G140C and V151I) compared with subtype B, and one position in which subtypes A1 and B displayed a higher genetic barrier (L74M and L74I) than sub-subtype A6. Additionally, we confirmed that the L74I mutation was selected at the early stage of the epidemic and subsequently spread as a founder effect in Russia. Our data have added to the overall understanding of the genetic features of sub-subtype A6 in the context of drug resistance. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Συγγραφείς:
Kirichenko, A.
Lapovok, I.
Baryshev, P.
van de Vijver, D.A.M.C.
van Kampen, J.J.A.
Boucher, C.A.B.
Paraskevis, D.
Kireev, D.
Περιοδικό:
Virus Bulletin
Λέξεις-κλειδιά:
integrase; integrase; integrase inhibitor, adult; amino acid sequence; Article; gene mutation; gene sequence; gene structure; genetic analysis; genetic distance; genetic variability; genetic variation; genotype; geographic distribution; human; Human immunodeficiency virus infection; major clinical study; nucleotide sequence; phylogenetic tree; phylogeny; phylogeography; RNA extraction; RNA sequence; Russian (people); antiviral resistance; classification; DNA sequence; drug effect; enzymology; female; genetic polymorphism; genetics; Human immunodeficiency virus 1; Human immunodeficiency virus infection; male; mutation; prevalence; Russian Federation, Adult; Drug Resistance, Viral; Female; Genotype; HIV Infections; HIV Integrase; HIV Integrase Inhibitors; HIV-1; Humans; Male; Mutation; Phylogeny; Polymorphism, Genetic; Prevalence; Russia; Sequence Analysis, DNA
