Τίτλος:
Differential gene expression profiling of Listeria monocytogenes in cacciatore and felino salami to reveal potential stress resistance biomarkers
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The current study reports a) the in situ transcriptional profiles of Listeria monocytogenes in response to fermented sausage stress and b) an approach in which in situ RT-qPCR data have been combined with advanced statistical techniques to discover potential stress resistance or cell viability biomarkers. Gene expression profiling of the pathogen has been investigated using RT-qPCR to understand how L.monocytogenes responds to the conditions encountered during the fermentation and ripening of sausages. A cocktail of five L.monocytogenes strains was inoculated into the batter of Cacciatore and Felino sausages. The RT-qPCR data showed that the acidic and osmotic stress-related genes were up-regulated. The transcripts of the lmo0669 gene increased during the fermentation and ripening of Cacciatore, whereas gbuA and lmo1421 were up-regulated during the ripening of Felino and Cacciatore, respectively. sigB expression was induced in both sausages throughout the whole process. Finally, the virulence-related gene prfA was down-regulated during the fermentation of Cacciatore. The multivariate gene expression profiling analysis suggested that sigB and lmo1421 or sigB and gbuA could be used as different types of stress resistance biomarkers to track, for example, stress resistance or cell viability in fermented sausages with short (Cacciatore) or long (Felino) maturation times, respectively. © 2014 Elsevier Ltd.
Συγγραφείς:
Mataragas, M.
Rovetto, F.
Bellio, A.
Alessandria, V.
Rantsiou, K.
Decastelli, L.
Cocolin, L.
Περιοδικό:
Food Microbiology
Εκδότης:
INSTAP Academic Press
Λέξεις-κλειδιά:
bacterial protein, analysis; animal; fermentation; food contamination; gene expression profiling; genetics; isolation and purification; Listeria monocytogenes; meat; metabolism; microbiology; physiological stress; physiology; pig, Animals; Bacterial Proteins; Fermentation; Food Contamination; Gene Expression Profiling; Listeria monocytogenes; Meat Products; Stress, Physiological; Swine
DOI:
10.1016/j.fm.2014.09.003
