Ερευνητικό υλικό ΕΚΠΑ

Differentiation of serratia marcescens and Serratia liquefaciens by tests for lipase and phospholipase production

Αγγλικά

The production of lipase and phospholipase by certain members of the Enterobacteriaceae was examined by thin-layer chromatography of resting-cell suspensions incubated with triolein or lecithin. Most strains of Serratia marcescens produced both enzymes, while most strains of Serratia liquefaciens exhibited strong lipase but only a minor phospholipase activity. Enterobacter spp. (25 strains), Klebsiella pneumoniae (20 strains), E. coli (15 strains), Citrobacter freundii (7 strains) and Proteus spp. (20 strains) lacked both types of enzymic activity except for the following: three strains of Enterobacter cloacae, two of Proteus mirabilis and three of Proteus vulgaris possessed slight lipase activity; about one-half of the Enterobacter aerogenes and Enterobacter hafniae strains examined produced slight phospholipase activity. It is suggested that tests for lipase and phospholipase should be used in conjunction with those for DNAase production and sugar fermentation for the differentiation of S. marcescens and S. liquefaciens.

1978

Legakis, N.J.
Nicolas, K.J.
Xilinas, M.
Papavassiliou, J.

Journal of Medical Microbiology

11

3

225-231

phospholipase; triacylglycerol lipase, animal experiment; classification; Enterobacteriaceae; in vitro study; Serratia liquefaciens; Serratia marcescens; taxonomy

https://doi.org/10.1099/00222615-11-3-225

10.1099/00222615-11-3-225

https://pergamos.lib.uoa.gr/uoa/dl/object/3002699