Τίτλος:
Therapeutic Effects of Mesenchymal Stem Cells Derived From Bone Marrow, Umbilical Cord Blood, and Pluripotent Stem Cells in a Mouse Model of Chemically Induced Inflammatory Bowel Disease
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Acute inflammatory bowel disease (AIBD) is a wide clinical entity including severe gastrointestinal pathologies with common histopathological basis. Epidemiologically increasing diseases, such as necrotizing enterocolitis (NEC), gastrointestinal graft versus host disease (GVHD), and the primary acute phase of chronic inflammatory bowel disease (CIBD), exhibit a high necessity for new therapeutic strategies. Mesenchymal stem cell (MSC) cellular therapy represents a promising option for the treatment of these diseases. In our study, we comparatively assess the efficacy of human MSCs derived from bone marrow (BM), umbilical cord blood (UCB), human embryonic stem cells (ESCs), or human-induced pluripotent stem cells (iPSCs) in a mouse model of chemically induced acute enterocolitis. The laboratory animals were provided ad libitum potable dextrane sulfate sodium solution (DSS) in order to reproduce an AIBD model and then individually exposed intraperitoneally to MSCs derived from BM (BM-MSCs), UCB (UCB-MSCs), ESCs (ESC-MSCs), or iPSCs (iPSC-MSCs). The parameters used to evaluate the cellular treatment efficacy were the animal survival prolongation and the histopathological-macroscopic picture of bowel sections. Although all categories of mesenchymal stem cells led to statistically significant survival prolongation compared to the control group, significant clinical and histopathological improvement was observed only in mice receiving BM-MSCs and UCB-MSCs. Our results demonstrated that the in vivo anti-inflammatory effect of ESC-MSCs and iPSC-MSCs was inferior to that of UCB-MSCs and BM-MSCs. Further investigation will clarify the potential of ESCs and iPSC-derived MSCs in AIBD treatment. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Συγγραφείς:
Kagia, A.
Tzetis, M.
Kanavakis, E.
Perrea, D.
Sfougataki, I.
Mertzanian, A.
Varela, I.
Dimopoulou, A.
Karagiannidou, A.
Goussetis, E.
Περιοδικό:
Journal of Inflammation Research
Εκδότης:
Springer New York LLC
Λέξεις-κλειδιά:
dextran sulfate, acute disease; adult; animal experiment; animal model; antiinflammatory activity; Article; bone marrow derived mesenchymal stem cell; chemically induced disorder; controlled study; embryo; enterocolitis; fetus; histopathology; human; human cell; human embryonic stem cell; in vivo study; induced pluripotent stem cell; inflammatory bowel disease; mesenchymal stem cell; mouse; nonhuman; therapy effect; umbilical cord blood; animal; bone marrow cell; cytology; disease model; fetus blood; induced pluripotent stem cell; inflammatory bowel disease; mesenchymal stem cell transplantation; mortality; procedures; survival analysis, Animals; Bone Marrow Cells; Disease Models, Animal; Fetal Blood; Humans; Induced Pluripotent Stem Cells; Inflammatory Bowel Diseases; Mesenchymal Stem Cell Transplantation; Mice; Survival Analysis
DOI:
10.1007/s10753-019-01033-x
