Τίτλος:
Molecular epidemiology of noroviruses in children in South Greece, 2013-2015
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Noroviruses constitute the leading cause of acute, nonbacterial gastroenteritis that affects both children and adults in healthcare and community settings. The current study attempted to provide insight on the molecular epidemiology of noroviruses in children in South Greece. Genotypic characterization of 69 norovirus strains detected in stool samples from children with gastroenteritis during a period of 30 months (January 2013 to June 2015) was performed on the basis of ORF2 (VP1 capsid) gene sequences. The results revealed the circulation of a diverse variety of norovirus genotypes. GII.4 was the predominant genotype (74%), followed by GII.2 (8.7%), GII.3 (5.8%), GII.6 (2.9%), GI.2 (2.9%), and four strains identified as GII.1, GII.7, GII.8, and GII.13, respectively. Phylogenetic analysis showed that most of the strains were closely associated with norovirus strains that circulated globally either in outbreaks and sporadic cases of gastroenteritis or in the environment during the last 4 years. Οf the GII.4 strains, 80.4% were detected between January 2013 and February 2014, indicating a possible ongoing epidemic. The incidence of other genotypes remained constant throughout the study period. Genotypic and phylogenetic analysis showed the predominance of the “Sydney 2012” variant among the GII.4 strains, whereas one GII.4 strain was identified as a “New Orleans 2009” variant. Five GII.4 strains showed significant nucleotide and amino acid sequence divergence from either the “Sydney 2012” or the “New Orleans 2009” variant, and these divergent strains might represent an emerging GII.4 variant. © 2018 Wiley Periodicals, Inc.
Συγγραφείς:
Siafakas, N.
Zerva, L.
Hatzaki, D.
Lebessi, E.
Chronopoulou, G.
Paraskakis, I.
Pournaras, S.
Περιοδικό:
Journal of Medical Virology
Εκδότης:
John Wiley and Sons Inc
Λέξεις-κλειδιά:
protein VP1; capsid protein, adolescent; amino acid sequence; Article; child; disease duration; female; genetic analysis; genetic variability; genotype; Greece; human; infant; major clinical study; male; microscopy; molecular epidemiology; newborn; nonhuman; Norovirus; nucleotide sequence; ORF2 gene; phylogeny; preschool child; school child; viral gastroenteritis; virus characterization; virus detection; virus gene; virus strain; calicivirus infection; classification; DNA sequence; feces; genetic variation; genetics; genotype; genotyping technique; incidence; isolation and purification; molecular epidemiology; Norovirus; virology, Adolescent; Caliciviridae Infections; Capsid Proteins; Child; Child, Preschool; Feces; Female; Genetic Variation; Genotype; Genotyping Techniques; Greece; Humans; Incidence; Infant; Infant, Newborn; Male; Molecular Epidemiology; Norovirus; Phylogeny; Sequence Analysis, DNA
