Τίτλος:
Impact of deserosalization on small bowel anastomosis healing in swine: A pilot study
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Healing is related to gastrointestinal anastomotic leak, which is a severe and common complication. This study aimed to investigate the feasibility and the impact of deserosalization on healing of jejuno-jejunal anastomoses in an animal model. Materials and Methods: Seven swine underwent three types of side-to-side jejuno-jejunal anastomosis twice and survived seven days. Three different types of jejuno-jejunal sideto- side anastomoses were performed twice at 20-cm distance from each other in each animal: no serosa removal, one-sided, and two-sided serosa removal, respectively. Bursting pressure, tissue hydroxyproline concentration, and pathology scores were evaluated. Results: Hydroxyproline tissue concentration was a mean±standard deviation of 0.37±0.09, 0.38±0.08, and 0.30±0.05 nmoI/ml respectively (p<0.05). Bursting pressure was a mean±standard deviation of 59.02±8.60, 73.20±11.09, and 100.01±7.49 mmHg, respectively (p<0.001). The histopathological assessment did not find any statistically significant differences. Conclusion: Deserosalization in jejunojejunal anastomosis was technically feasible and seemed to improve mechanical strength and collagen deposition in this experimental porcine model. Further investigation is warranted. © 2020 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Vrakopoulou, G.-Z.
Toutouzas, K.G.
Giannios, P.
Panousopoulos, S.-G.
Theodoropoulos, C.
Danas, E.
Liakea, A.
Papalois, A.E.
Zografos, G.
Larentzakis, A.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
hydroxyproline; collagen, animal experiment; animal model; animal tissue; Article; chemical reaction; controlled study; deserosalization; feasibility study; female; histopathology; intestine anastomosis; jejuno jejunal side to side anastomosis; jejunum; Landrace pig; nonhuman; pilot study; serosa; statistically significant result; wound healing; anastomosis; animal; colon; pig; small intestine; surgery, Anastomosis, Surgical; Animals; Collagen; Colon; Intestine, Small; Pilot Projects; Swine; Wound Healing
DOI:
10.21873/invivo.12056
