Τίτλος:
Deep sequencing identified dysregulated circulating MicroRNAs in late onset Preeclampsia
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop lateonset preeclampsia (LOPE) compared to uncomplicated pregnancies. Materials and Methods: Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls. Results: miR-23b-5p and miR- 99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed that the major targets of these miRNAs are associated with glycometabolism and immune response. Conclusion: miR-23b-5p and miR-99b-5p are possibly implicated in the pathogenic mechanisms leading to the induction of LOPE and may serve as candidate noninvasive biomarkers for early prediction and prevention. © 2020 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Mavreli, D.
Lykoudi, A.
Lambrou, G.
Papaioannou, G.
Vrachnis, N.
Kalantaridou, S.
Papantoniou, N.
Kolialexi, A.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
circulating microRNA; glucagon; glycosaminoglycan; insulin; microRNA 23b 5p; microRNA 99b 5p; T lymphocyte receptor; unclassified drug; biological marker; circulating microRNA; microRNA, adult; Article; case control study; clinical article; controlled study; down regulation; female; first trimester pregnancy; gene ontology; high throughput sequencing; human; human tissue; immune response; insulin resistance; insulin signaling; maternal plasma; osteoclastogenesis; preeclampsia; pregnancy outcome; real time polymerase chain reaction; retrospective study; RNA sequencing; upregulation; gene expression profiling; genetics; high throughput sequencing; preeclampsia; pregnancy, Biomarkers; Circulating MicroRNA; Female; Gene Expression Profiling; High-Throughput Nucleotide Sequencing; Humans; MicroRNAs; Pre-Eclampsia; Pregnancy
DOI:
10.21873/invivo.12044
