Τίτλος:
Evaluation of cadmium-induced transcriptome alterations by three color cDNA labeling microarray analysis on a T-cell line
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Beside heavy metals, cadmium (Cd2+) is a ubiquitous toxic metal with a well established apoptotic and genotoxic effect, chronic exposure of which has been involved in a variety of pathological conditions. In the present study, we investigated by 1455 genes cDNA microarrays the toxic and apoptotic effect of Cd2+, on the T-cell line CCRF-CEM, applying a three laser differential analysis, on the same microarray slide. The cells were cultured for 6 and 24 h in the absence (control) or presence of Cd2+ (10 or 20 μM), RNAs were extracted and the produced cDNAs were labeled with rhodamine derivatives fluorescent dyes. A microarray slide was simultaneously hybridized by the labeled cDNAs and analyzed. We found that, in relation to control, treatment of the cells for 6 h with 10 and 20 μM Cd2+, induces up-regulation in 20 and 34 genes, respectively. Treatment for 24 h with 10 and 20 μM Cd2+ induces up-regulation in 22 and 84 genes, respectively. Twenty-eight genes were found down-regulated only after treatment for 24 h with Cd2+ 10 μM. These data suggest that Cd2+ produces a time- and dose-dependent molecular cascade, induces disturbances in different subcellular compartments, influencing thereafter the normal cellular functions, the differentiation process, the malignant transformation and the cell death. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Συγγραφείς:
Tsangaris, G.Th.
Botsonis, A.
Politis, I.
Tzortzatou-Stathopoulou, F.
Περιοδικό:
EMC - Toxicologie-Pathologie
Λέξεις-κλειδιά:
cadmium; complementary DNA; fluorescent dye; RNA, animal cell culture; apoptosis; article; biotransformation; cell death; cell function; cell line; controlled study; DNA determination; DNA microarray; down regulation; evaluation; human; human cell; labeling index; nucleotide sequence; priority journal; RNA extraction; T lymphocyte; unindexed sequence, Apoptosis; Blotting, Northern; Cadmium; Cell Survival; Culture Media; DNA, Complementary; Fluorescent Dyes; Immunohistochemistry; In Situ Hybridization, Fluorescence; Indicators and Reagents; Lasers; Mutagens; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; T-Lymphocytes; Up-Regulation
DOI:
10.1016/S0300-483X(02)00236-6
