Τίτλος:
Carbon nanohorn/liposome systems: Preformulation, design and in vitro toxicity studies
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
In the present work, the convergence of two different drug delivery systems is investigated, namely the combination of carbon nanohorns (CNHs) and liposomes. Our effort initially included the synthesis of two conversely charged carbon nanohorns and their subsequent analysis through various methods. The study of their effect on the thermotropic behavior of artificial membranes provided an essential assistance for the upcoming liposome preparation, which were estimated for their physicochemical properties. The presence of CNHs alters the calorimetric parameters of the lipids. We also prepared CNHs:liposome systems. The characteristic morphology and secondary spherical superstructure of CNHs is retained in the chimeric materials, suggesting that the interactions with the liposomes do not alter the dahlia-flower-like aggregation of CNHs. Both CNHs-liposome systems exhibit a relatively small cellular cytotoxicity in vitro, tested in mouse embryonic fibroblasts. To summarize, we developed CNHs:liposome platforms with a complete knowledge of their thermotropic, physicochemical, morphological and nanotoxicological characteristics. © 2019 Elsevier B.V.
Συγγραφείς:
Pippa, N.
Stangel, C.
Kastanas, I.
Triantafyllopoulou, E.
Naziris, N.
Stellas, D.
Zhang, M.
Yudasaka, M.
Demetzos, C.
Tagmatarchis, N.
Περιοδικό:
Materials Science and Engineering C: Biomimetic and Supramolecular Systems
Εκδότης:
Elsevier Ireland Ltd
Λέξεις-κλειδιά:
Carbon; Cell culture; Differential scanning calorimetry; Liposomes; Nanohorns; Physicochemical properties; Toxicity; Transmission electron microscopy, Artificial membranes; Carbon nanohorn; Cellular cytotoxicity; Drug delivery system; In-vitro; Mouse embryonic fibroblasts; Pre formulations; Thermotropic behavior, Drug delivery, carbon; lipid; liposome; nanomaterial, animal; C57BL mouse; cell survival; chemistry; cytology; drug formulation; fibroblast; lipid bilayer; mammalian embryo; temperature; three-dimensional imaging; toxicity testing; ultrastructure, Animals; Carbon; Cell Survival; Drug Compounding; Embryo, Mammalian; Fibroblasts; Imaging, Three-Dimensional; Lipid Bilayers; Lipids; Liposomes; Mice, Inbred C57BL; Nanostructures; Temperature; Toxicity Tests
DOI:
10.1016/j.msec.2019.110114
