Τίτλος:
Comparative evaluation of the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA)
assays and the AMPLICOR HIV-1 MONITOR v1.5 test for the quantitation of
human immunodeficiency virus type 1 RNA in plasma
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
HIV-1 RNA measurements from 84 plasma specimens obtained with the
QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays (Chiron Diagnostics,
Emeryville, CA) and with the AMPLICOR HIV-1 MONITOR (TM) Test, version
1.5 with ultra-sensitive specimen preparation (Roche Diagnostic Systems,
Inc., Branchburg, NJ) were compared. The absolute RNA values of tested
specimens differed significantly between bDNA 2.0 and bDNA 3.0 or
Monitor v1.5 measurements (Wilcoxon signed-rank test P < 0.001). Results
generated with bDNA 3.0 or with Monitor v1.5 were approximately twofold
greater than those generated with bDNA 2.0, with smaller differences at
higher HIV-1 RNA levels and greater differences at RNA levels below 1000
copies per mi. Although highly correlated (r = 0.92 and 0.86,
respectively), viral load data generated with bDNA 2.0 and either bDNA
3.0 or Monitor v1.5 were in poor agreement. Concordant results
(difference in log(10) copies per ml < 0.5) were found at frequencies of
80% for bDNA 2.0 and bDNA 3.0 and only at 58.5% for bDNA 2.0 and
Monitor v1.5. In contrast, bDNA 3.0 and Monitor v1.5 measurements were
highly correlated (r = 0.96) and in good agreement (92.7%). (C) 2001
Elsevier Science B.V. All rights reserved.
Συγγραφείς:
Anastassopoulou, CG
Toulomi, G
Katsoulidou, A and
Hatzitheodorou, H
Pappa, M
Paraskevis, D
Lazanas, M and
Gargalianos, P
Hatzakis, A
Περιοδικό:
Journal of Virological Methods
Εκδότης:
ELSEVIER SCIENCE BV
Λέξεις-κλειδιά:
HIV-1 RNA; viral load; branched DNA; Amplicor
DOI:
10.1016/S0166-0934(00)00245-7
