Τίτλος:
Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Sepsis is a dysregulated host response to infection related to devastating outcomes. Recently, interest has been shifted towards apoptotic and antiapoptotic pathobiology. Apoptosis is executed through the activation of caspases regulated by a number of antiapoptotic proteins, such as survivin. The survivin and caspases’ responses to sepsis have not yet been elucidated. This is a multicenter prospective observational study concerning patients with sepsis (n = 107) compared to patients with traumatic systemic inflammatory response syndrome (SIRS) (n = 75) and to healthy controls (n = 89). The expression of survivin was quantified through real-time quantitative polymerase chain reaction for the different survivin splice variants (wild type-WT, ΔEx3, 2B, 3B) in peripheral blood leukocytes. The apoptotic or antiapoptotic tendency was specified by measuring survivin-WT, caspase-3, and -9 serum protein concentrations through enzyme-linked immunosorbent assay. The survivin-WT, -2B, -ΔΕx3 mRNA, survivin protein, and caspases showed an escalated increase in SIRS and sepsis, whereas survivin-3B was repressed in sepsis (p < 0.05). Survivin correlated with IL-8 and caspase-9 (p < 0.01). For discriminating sepsis, caspase-9 achieved the best receiver operating characteristic curve (AUROC) of 0.95. In predicting mortality, caspase-9 and survivin protein achieved an AUROC of 0.70. In conclusion, specific apoptotic and antiapoptotic pathways might represent attractive targets for future research in sepsis. © 2021, The Author(s).
Συγγραφείς:
Miliaraki, M.
Briassoulis, P.
Ilia, S.
Polonifi, A.
Mantzourani, M.
Briassouli, E.
Vardas, K.
Nanas, S.
Pistiki, A.
Theodorakopoulou, M.
Tavladaki, T.
Spanaki, A.M.
Kondili, E.
Dimitriou, H.
Tsiodras, S.
Georgopoulos, D.
Armaganidis, A.
Daikos, G.
Briassoulis, G.
Περιοδικό:
Scientific Reports
Εκδότης:
Institute of Geographic Sciences and Natural Resources Research
Λέξεις-κλειδιά:
baculoviral IAP repeat containing protein 5; BIRC5 protein, human; caspase; caspase 3; caspase 9; messenger RNA, blood; case control study; clinical trial; female; human; male; metabolism; middle aged; mortality; multicenter study; prospective study; real time polymerase chain reaction; sepsis; systemic inflammatory response syndrome, Case-Control Studies; Caspase 3; Caspase 9; Caspases; Female; Humans; Male; Middle Aged; Prospective Studies; Real-Time Polymerase Chain Reaction; RNA, Messenger; Sepsis; Survivin; Systemic Inflammatory Response Syndrome
DOI:
10.1038/s41598-020-78208-2
