Τίτλος:
Targeted Downregulation of s36 Protein Unearths its Cardinal Role in Chorion Biogenesis and Architecture during Drosophila melanogaster Oogenesis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Drosophila chorion represents a model biological system for the in vivo study of gene activity, epithelial development, extracellular-matrix assembly and morphogenetic-patterning control. It is produced during the late stages of oogenesis by epithelial follicle cells and develops into a highly organized multi-layered structure that exhibits regional specialization and radial complexity. Among the six major proteins involved in chorion's formation, the s36 and s38 ones are synthesized first and regulated in a cell type-specific and developmental stage-dependent manner. In our study, an RNAi-mediated silencing of s36 chorionic-gene expression specifically in the follicle-cell compartment of Drosophila ovary unearths the essential, and far from redundant, role of s36 protein in patterning establishment of chorion's regional specialization and radial complexity. Without perturbing the developmental courses of follicle-and nurse-cell clusters, the absence of s36 not only promotes chorion's fragility but also induces severe structural irregularities on chorion's surface and entirely impairs fly's fertility. Moreover, we herein unveil a novel function of s36 chorionic protein in the regulation of number and morphogenetic integrity of dorsal appendages in follicles sporadically undergoing aged fly-dependent stress. © 2016 The Author(s).
Συγγραφείς:
Velentzas, A.D.
Velentzas, P.D.
Sagioglou, N.E.
Konstantakou, E.G.
Anagnostopoulos, A.K.
Tsioka, M.M.
Mpakou, V.E.
Kollia, Z.
Consoulas, C.
Margaritis, L.H.
Papassideri, I.S.
Tsangaris, G.T.
Sarantopoulou, E.
Cefalas, A.-C.
Stravopodis, D.J.
Περιοδικό:
Scientific Reports
Εκδότης:
Nature Publishing Group
Λέξεις-κλειδιά:
biogenesis; chorion; dorsal region; down regulation; Drosophila melanogaster; fertility; gene expression regulation; gene inactivation; gene silencing; human; nonhuman; nurse; oocyte development; ovary follicle cell; RNA interference; specialization; stress; aging; animal; chorion; down regulation; Drosophila melanogaster; female; genetics; metabolism; oocyte development; ovary follicle; physiology; transgenic animal, chorion proteins; Drosophila protein; egg protein; small interfering RNA, Aging; Animals; Animals, Genetically Modified; Chorion; Down-Regulation; Drosophila melanogaster; Drosophila Proteins; Egg Proteins; Female; Gene Expression Regulation, Developmental; Oogenesis; Ovarian Follicle; RNA, Small Interfering
