Τίτλος:
Uptake of BSA-FITC loaded PLGA nanoparticles by bone marrow-derived dendritic cells induces maturation but not IL-12 or IL-10 production
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Nanoparticles prepared from biodegradable polymers, such as poly(lactide-co-glycolide) (PLGA), represent a new approach for vaccine delivery due to their ability to be taken up by phagocytes and activate immune responses. In this study, fluorescently labelled bovine serum albumin (BSA-FITC)-loaded PLGA nanoparticles, of an average size ∼300 nm were prepared and examined for their ability to be taken up by bone marrow-derived dendritic cells (BM-DCs) in vitro and thus to promote their maturation and activation. The synthesized nanoparticles did not exhibit any cytotoxic or hemolytic effect and were taken up by BM-DCs efficiently, in a time and dose dependent manner. The localization of BSA-FITC loaded PLGA nanoparticles both in the acidophilic cellular compartments and the cytoplasm resulted in the maturation of BM-DCs expressing higher levels of costimulatory and MHC class II molecules in comparison to empty PLGA nanoparticles. However, the absence of IL-12 or IL-10 production indicates partial activation of BM-DCs suggesting the necessity of an adjuvant addition in order to facilitate DCs functionalization. Copyright © 2013 American Scientific Publishers. All rights reserved.
Συγγραφείς:
Karagouni, E.
Kammona, O.
Margaroni, M.
Kotti, K.
Karageorgiou, V.
Gaitanaki, C.
Kiparissides, C.
Περιοδικό:
Nanoscience and Nanotechnology Letters
Λέξεις-κλειδιά:
BSA-FITC; DCs maturation; IL-10; IL-12; PLGA nanoparticles, Bone; Cells; Copolymers; Cytology; Nanoparticles, Synthesis (chemical)
DOI:
10.1166/nnl.2013.1564
