Τίτλος:
In vitro digestibility of β-casein and β-lactoglobulin under simulated human gastric and duodenal conditions: A multi-laboratory evaluation
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Initially the resistance to digestion of two cow's milk allergens, β-casein, and β-lactoglobulin (β-Lg), was compared using a "high-protease assay" and a "low-protease assay" in a single laboratory. The low-protease assay represents an alternative standardised protocol mimicking conditions found in the gastrointestinal tract. For the high-protease assay, both proteins were incubated with either pepsin or pancreatin and digestion monitored by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse phase-high performance liquid chromatography. The low-protease assay involved gastroduodenal digestion in the presence or absence of phosphatidylcholine (PC). Both β-casein and β-Lg were susceptible to hydrolysis by pepsin and pancreatin in the high-protease assay. In contrast, the kinetics of β-casein digestion in the low-protease assay were slower, β-Lg being pepsin resistant. During duodenal digestion, β-Lg was gradually degraded and addition of PC slowed digestion. Subsequently, the reproducibility of the low-protease assay was assessed in 12 independent laboratories by visual assessment of the gels and densitometric analysis: the inter- and intra-laboratory variability was affected by sampling and electrophoresis method employed. The low-protease assay was shown to be reproducible. Future studies will extend these findings using a broader panel of proteins. © 2009 Elsevier Inc.
Συγγραφείς:
Mandalari, G.
Adel-Patient, K.
Barkholt, V.
Baro, C.
Bennett, L.
Bublin, M.
Gaier, S.
Graser, G.
Ladics, G.S.
Mierzejewska, D.
Vassilopoulou, E.
Vissers, Y.M.
Zuidmeer, L.
Rigby, N.M.
Salt, L.J.
Defernez, M.
Mulholland, F.
Mackie, A.R.
Wickham, M.S.J.
Mills, E.N.C.
Περιοδικό:
Regulatory Toxicology and Pharmacology
Λέξεις-κλειδιά:
beta casein; beta lactoglobulin; pancreatin; pepsin A; phosphatidylcholine, allergy; article; controlled study; densitometry; digestion; duodenum; electrophoresis; enzyme assay; food safety; in vitro study; laboratory test; nonhuman; nucleotide sequence; polyacrylamide gel electrophoresis; priority journal; protein hydrolysis; reproducibility; reversed phase high performance liquid chromatography; simulation; stomach, Allergens; Animals; Caseins; Chromatography, High Pressure Liquid; Digestion; Duodenum; Electrophoresis, Polyacrylamide Gel; Humans; Lactoglobulins; Milk; Pancreatin; Pepsin A; Reproducibility of Results; Sodium Dodecyl Sulfate; Stomach
DOI:
10.1016/j.yrtph.2009.08.010
