Τίτλος:
Validated GC/MS method for the simultaneous determination of clozapine and norclozapine in human plasma. Application in psychiatric patients under clozapine treatment
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A sensitive and specific GC/MS method for the determination of clozapine (CLZ) and its major metabolite norclozapine (NCLZ), in plasma has been developed, optimized and validated. Specimen preparation includes solid-phase extraction of both analytes using Bond-Elut Certify cartridge and further derivatization with TFAA. Clozapine-d8 was used as internal standard for the determination of CLZ and NCLZ. Limits of detection were 0.45. ng/mL for CLZ and 1.59. ng/mL for NCLZ, while limits of quantification were 1.37. ng/mL for CLZ and 4.8. ng/mL for NCLZ, as calculated by the calibration curves. The calibration curves were linear up to 600. ng/mL for CLZ and NCLZ. Absolute recovery ranged from 82.22% to 95.35% for both analytes. Intra- and interday accuracy was less than 7.13% and -12.52%, respectively, while intra- and interday precision was between 9.47% and 12.07%, respectively, for CLZ and NCLZ. The method covers all therapeutic range and proved suitable for the determination of CLZ and NCLZ not only in psychiatric patients but also in forensic cases with clozapine implication. © 2010 Elsevier B.V.
Συγγραφείς:
Vardakou, I.
Dona, A.
Pistos, C.
Alevisopoulos, G.
Athanaselis, S.
Maravelias, C.
Spiliopoulou, C.
Περιοδικό:
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Λέξεις-κλειδιά:
Analytes; Calibration curves; Clozapine; Derivatizations; GC/MS; Human plasmas; Internal standards; Limits of detection; Limits of quantification; Norclozapine; Patients; Psychiatric patients; Simultaneous determinations; Solid-phase extraction; Therapeutic range, Calibration; Extraction; Specimen preparation, Plasmas, clozapine; norclozapine, accuracy; article; drug blood level; drug determination; drug metabolism; gas chromatography; human; mass spectrometry; mental patient; priority journal; schizophrenia; solid phase extraction, Clozapine; Gas Chromatography-Mass Spectrometry; Humans; Linear Models; Mental Disorders; Reproducibility of Results; Sensitivity and Specificity; Solid Phase Extraction
DOI:
10.1016/j.jchromb.2010.07.001
