Τίτλος:
Chimeric stimuli-responsive liposomes as nanocarriers for the delivery of the anti-glioma agent TRAM-34
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Nanocarriers are delivery platforms of drugs, peptides, nucleic acids and other therapeutic molecules that are indicated for severe human diseases. Gliomas are the most frequent type of brain tumor, with glioblastoma being the most common and malignant type. The current state of glioma treatment requires innovative approaches that will lead to efficient and safe therapies. Advanced nanosystems and stimuli-responsive materials are available and well-studied technologies that may contribute to this effort. The present study deals with the development of functional chimeric nanocarriers composed of a phospholipid and a diblock copolymer, for the incorporation, delivery and pH-responsive release of the antiglioma agent TRAM-34 inside glioblastoma cells. Nanocarrier analysis included light scattering, protein incubation and electron microscopy, and flu-orescence anisotropy and thermal analysis techniques were also applied. Biological assays were carried out in order to evaluate the nanocarrier nanotoxicity in vitro and in vivo, as well as to evaluate antiglioma activity. The nanosystems were able to successfully manifest functional properties under pH conditions, and their biocompatibility and cellular internalization were also evident. The chimeric nanoplatforms presented herein have shown promise for biomedical applications so far and should be further studied in terms of their ability to deliver TRAM-34 and other therapeutic molecules to glioblastoma cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Naziris, N.
Pippa, N.
Sereti, E.
Chrysostomou, V.
Kędzierska, M.
Kajdanek, J.
Ionov, M.
Miłowska, K.
Balcerzak, Ł.
Garofalo, S.
Limatola, C.
Pispas, S.
Dimas, K.
Bryszewska, M.
Demetzos, C.
Περιοδικό:
International Journal of Molecular Sciences
Λέξεις-κλειδιά:
1 [(2 chlorophenyl)diphenylmethyl] 1h pyrazole; copolymer; fluorescent dye; lactate dehydrogenase; liposome; nanocarrier; phosphatidylcholine; phospholipid; poly(lauryl methacrylate); poly[2 (dimethylamino)ethyl methacrylate]; rhodamine B; unclassified drug; 1 [(2 chlorophenyl)diphenylmethyl] 1h pyrazole; drug carrier; liposome; nanoparticle; polymer; pyrazole derivative, acute toxicity; animal cell; animal experiment; animal model; animal tissue; anisotropy; antineoplastic activity; antiproliferative activity; Article; bioassay; biocompatibility; chemical analysis; clinical evaluation; controlled study; drug release; electron microscopy; fluorescence analysis; genotoxicity; GL261 cell line; glioblastoma; glioblastoma cell line; human; human cell; immunotoxicity; in vitro study; in vivo study; incubation time; internalization (cell); light scattering; liposomal delivery; liposome membrane; male; membrane fluidity; mouse; nanotoxicology; nonhuman; pH; phase transition; protein interaction; thermal analysis; ultraviolet visible spectroscopy; apoptosis; cell proliferation; chemistry; drug delivery system; glioma; metabolism; pathology; tumor cell culture, Apoptosis; Cell Proliferation; Drug Carriers; Drug Delivery Systems; Glioma; Humans; Hydrogen-Ion Concentration; Liposomes; Nanoparticles; Polymers; Pyrazoles; Tumor Cells, Cultured
DOI:
10.3390/ijms22126271
