Τίτλος:
Biological effect of silver-modified nanostructured titanium dioxide in cancer
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2NPs) with anticancer potential. Materials and Methods: Ag/TiO2NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO2NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions. © 2021 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Lagopati, N.
Kotsinas, A.
Veroutis, D.
Evangelou, K.
Papaspyropoulos, A.
Arfanis, M.
Falaras, P.
Kitsiou, P.V.
Pateras, I.
Bergonzini, A.
Frisan, T.
Kyriazis, S.
Tsoukleris, D.S.
Tsilibary, E.-P.C.
Gazouli, M.
Pavlatou, E.A.
Gorgoulis, V.G.
Περιοδικό:
Cancer Genomics and Proteomics
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
silver nanoparticle; titanium dioxide nanoparticle; nanomaterial; silver; titanium; titanium dioxide, antineoplastic activity; apoptosis; Article; biological activity; cell culture; cell viability; colorimetry; cytotoxicity; DNA fragmentation assay; human; human cell; MCF-7 cell line; MDA-MB-231 cell line; MTT assay; photoactivation; protein expression; sol-gel; Western blotting; chemistry; nanomedicine; neoplasm; procedures, Humans; Nanomedicine; Nanostructures; Neoplasms; Silver; Titanium
