Τίτλος:
Up-regulation of a novel mRNA (NY-CO-1) involved in the methyl
4-methoxy-3-(3-methyl-2-butenoyl) benzoate (VT1)-induced proliferation
arrest of a non-small-cell lung carcinoma cell line (NSCLC-N6)
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
It is now well known that treatment of tumors, especially non-small-cell
lung cancer (NSCLC), remains limited and it is urgent to develop
strategies that target tumor cells and their genetic features. In this
regard, our work is about genetic modifications arising in an in vitro
NSCLC cell line after treatment with a chemical substance, methyl
4-methoxy-3-(3-methyl-2-butenoyl) benzoate (VT1). First, we showed that
VT1 induces arrest of proliferation by blocking cells in the GI phase of
the cell cycle. Second, we use “differential display” strategy to
clarify the genetic mechanisms involved in this proliferation arrest. A
novel mRNA, NY-CO-1 (New-York Colon 1), of unknown function showed
up-regulated expression after treatment. Application of “antisense”
strategy confirmed this novel mRNA induction was effectively linked to
growth arrest. Therefore, these data provide new information about
mechanisms participating in arrest of proliferation of tumor cells and
open new ways of treatment to target tumor growth. (C) 2001 Wiley-Liss.
Inc.
Συγγραφείς:
Carbonnelle, D
Jacquot, C
Lanco, X
Le Dez, G
Tomasoni, C
and Briand, G
Tsotinis, A
Calogeropoulou, T
Roussakis, C
Περιοδικό:
International Journal of Cancer
Λέξεις-κλειδιά:
bronchopulmonary carcinoma; NSCLC-N6 gene expression; VT1; NY-CO-1;
differential display
