Τίτλος:
Changes in HNK-1 epitope and collagen type IX in the aqueous humour of
patients with pseudoexfoliation syndrome
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Purpose. To investigate alterations in the proteoglycan (PG) and
glycosaminoglycan (GAG) content of the aqueous humour in patients with
pseudoexfoliation syndrome (PEX).
Materials and methods. Aqueous humor samples were obtained during
cataract surgery from nineteen patients bearing PEX features and
twenty-three age-matched normal controls. Protein and IgG were
quantified densitometrically after their electrophoretic separation.
Collagen type IX, 3-sulphoglucuronic acid (HNK-1 epitope), biglycan and
heparan sulphate proteoglycans were detected in Western and dot blots by
using specific monoclonal antibodies (MAbs). The immunochemical analysis
was; performed in native aqueous humour or after degradation of the
glycosaminoglycans with chondroitinases.
Results. Degradation of the samples with chondroitinases ABC, AC and B
revealed that, in the aqueous humour from PEX eyes, collagen type IX and
biglycan had a more dermatan sulphate than did normal eyes. In addition,
more HNK-1 epitope was observed in PEX eyes, which after similar
enzymatic treatment was found to be, located mainly in dermatan sulphate
sequences. 3-sulphoglucuronic acid was a constituent of the GAG chains
of the collagen type IX. We found that the electrophoretic mobility of
the bands of collagen type IX and HNK-1 epitope was exactly the same in
the aqueous humour of normal and PEX samples; both migrated as four
bands at 120, 113, 92.6 and 56 kDa. The PGs bearing heparan sulphate
were found only in normal samples. Other PGs were not detected.
Conclusion. Because no significant difference was observed in the
concentration of albumin and IgG in PEX and normal samples, the
blood-aqueous barrier was probably not significantly compromised in PEX
patients with cataract but without open-angle glaucoma. The results
support the hypothesis that the pathogenesis of PEX can be linked to
disturbed metabolism of GAGS and PGs.
Συγγραφείς:
Gartaganis, SP
Georgakopoulos, CD
Assouti, M
Mela, EK and
Exarchou, A
Giannelou, I
Gotsis, SS
Ziouti, N
Vynios, DH
and Tripathi, BJ
Tripathi, RC
Περιοδικό:
Current Eye Research
Εκδότης:
Taylor and Francis Inc.
Λέξεις-κλειδιά:
Western blotting; extracellular matrix; glycosaminoglycans; chondroitin
sulphate; dermatan sulphate
DOI:
10.1076/ceyr.28.1.5.23490
