Τίτλος:
The march of pluripotent stem cells in cardiovascular regenerative medicine
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Cardiovascular disease (CVD) continues to be the leading cause of global morbidity and mortality. Heart failure remains a major contributor to this mortality. Despite major therapeutic advances over the past decades, a better understanding of molecular and cellular mechanisms of CVD as well as improved therapeutic strategies for the management or treatment of heart failure are increasingly needed. Loss of myocardium is a major driver of heart failure. An attractive approach that appears to provide promising results in reducing cardiac degeneration is stem cell therapy (SCT). In this review, we describe different types of stem cells, including embryonic and adult stem cells, and we provide a detailed discussion of the properties of induced pluripotent stem cells (iPSCs). We also present and critically discuss the key methods used for converting somatic cells to pluripotent cells and iPSCs to cardiomyocytes (CMs), along with their advantages and limitations. Integrating and non-integrating reprogramming methods as well as characterization of iPSCs and iPSC-derived CMs are discussed. Furthermore, we critically present various methods of differentiating iPSCs to CMs. The value of iPSC-CMs in regenerative medicine as well as myocardial disease modeling and cardiac regeneration are emphasized. © 2018 The Author(s).
Συγγραφείς:
Abou-Saleh, H.
Zouein, F.A.
El-Yazbi, A.
Sanoudou, D.
Raynaud, C.
Rao, C.
Pintus, G.
Dehaini, H.
Eid, A.H.
Περιοδικό:
Stem Cell Research and Therapy
Εκδότης:
BioMed Central Ltd.
Λέξεις-κλειδιά:
CD34 antigen; cre recombinase; growth factor; messenger RNA; minicircle DNA; plasmid DNA; transcription factor NANOG; transposase; zinc finger nuclease, adipose derived stem cell; adult stem cell; cardiac stem cell; cardiomyopathy; cardiovascular disease; cell differentiation; cell nucleus transplantation; cell reprogramming technique; coculture; drug screening; embryoid body; embryonic stem cell; fibroblast; heart failure; heart pacing; human; induced pluripotent stem cell; keratinocyte; liquid chromatography-mass spectrometry; live cell imaging; long QT syndrome; LoxP site; melanocyte; multipotent stem cell; myocardial disease; neural stem cell; nonhuman; pluripotent stem cell; priority journal; Review; stem cell transplantation; tissue regeneration; transposon; umbilical cord blood; virus DNA cell DNA interaction; biological therapy; cardiac muscle cell; cardiovascular disease; genetics; metabolism; pluripotent stem cell; procedures; regenerative medicine, Cardiovascular Diseases; Cell Differentiation; Cell- and Tissue-Based Therapy; Humans; Myocytes, Cardiac; Pluripotent Stem Cells; Regenerative Medicine
DOI:
10.1186/s13287-018-0947-5
