Τίτλος:
Validation of a real-time quantitative polymerase chain reaction method for the quantification of 3 Survivin transcripts and evaluation in breast cancer tissues
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background Survivin is a novel antiapoptotic gene, which is a member of the inhibitor of apoptosis protein (IAP) family. Recently, 3 splice variants of this gene were cloned and characterized. This study aimed to validate a sensitive and specific method for the detection of survivin variants in breast cancer. Methods Real-time quantitative polymerase chain reaction (qPCR) was performed on the cDNA with a reverse primer specific for each splice variant and a pair of common hybridization probes. Results The expression of wild-type survivin was significantly correlated with survivin-2b, survivin-ΔEx3, and the ratio of survivin-ΔEx3 to wild-type survivin (P <.001). The ratio of survivin-2b to wild-type survivin was strongly associated with the ratio of survivin-ΔEx3 to wild-type survivin (P <.001). There was a strong positive association between the grade of the tumor and survivin-2b mRNA, survivin-ΔEx3 mRNA, and the ratio of survivin-ΔEx3 to wild-type survivin mRNA (P <.05). The ratio of survivin-2b to wild-type survivin was significantly associated with the presence of estrogen receptors (P =.05). Conclusion Our validated data suggest that survivin isoforms may be related to clinicopathological features and could be used as molecular prognostic tools or as new therapy targets. © 2014 Elsevier Inc. All rights reserved.
Συγγραφείς:
Pavlidou, A.
Kroupis, C.
Goutas, N.
Dalamaga, M.
Dimas, K.
Περιοδικό:
Clinical Breast Cancer
Εκδότης:
Cancer Information Group, LP
Λέξεις-κλειδιά:
estrogen receptor; messenger RNA; progesterone receptor; survivin; survivin 2b; survivin deltaex3; unclassified drug, agar gel electrophoresis; article; breast cancer; cancer grading; correlation analysis; disease association; human; human tissue; lymph node metastasis; protein expression; quantitative assay; real time polymerase chain reaction; real time quantitative polymerase chain reaction; sensitivity and specificity; tumor volume; validation study, Apoptosis; Dual hybridization probes; Prognostic biomarkers; qPCR; Survivin splice variants, Breast Neoplasms; Female; Gene Expression Regulation, Neoplastic; Humans; Inhibitor of Apoptosis Proteins; Neoplasm Grading; Neoplasm Invasiveness; Prognosis; Protein Isoforms; RNA Splicing; Survival Rate
DOI:
10.1016/j.clbc.2013.10.012
