Τίτλος:
Survivin messenger RNA levels in Epstein-Barr virus-positive patients with leukemic low-grade B-cell lymphomas expressing the latent membrane protein 1: Evidence of apoptotic function?
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background Epstein-Barr virus (EBV) is a ubiquitous pathogen that chronically infects B lymphocytes and is implicated in the pathogenesis of lymphoproliferative diseases. Latent membrane protein 1 (LMP1), the major oncoprotein of the virus, has been shown to inhibit apoptosis and trigger survivin expression in malignant cell lines. LMP1 expression has been detected in patients with chronic lymphocytic leukemia, but its properties have not been studied in patients with low-grade B-cell lymphomas. Recent data show that LMP1 can simultaneously induce and inhibit apoptosis in B cells. We detected LMP1 messenger RNA (mRNA) in patients with leukemic low-grade B-cell lymphoma and correlated the expression of the antiapoptotic molecule survivin to that of LMP1 in this group of patients. Patients and Methods Peripheral whole blood from 64 patients with low-grade B-cell lymphoma was tested by quantitative reverse transcriptase-polymerase chain reaction (PCR) for the presence of the BXLF-1 gene of EBV, and positive samples were tested by conventional PCR for LMP1 expression. Accordingly, survivin mRNA levels were measured by quantitative reverse transcriptase PCR in all samples and compared between LMP1-positive (LMP1+) and LMP1- patients. Results The BXLF-1 gene was detected in 27 of 64 patients (42%). LMP1 was expressed in 22 of 27 (81%) EBV+ patients. Survivin expression was found to be 6.36 times higher in LMP1- patients than in LMP1+ patients (P =.008). Conclusion Our results imply that in patients with non-EBV-related leukemic low-grade B-cell lymphoma, LMP1 expression is possibly correlated to apoptosis, as indicated by the lower survivin mRNA levels in LMP1+ patients. © 2014 Elsevier Inc. All rights reserved.
Συγγραφείς:
Diamantopoulos, P.T.
Polonyfi, K.
Sofotasiou, M.
Mantzourani, M.
Galanopoulos, A.
Spanakis, N.
Papadopoulou, V.
Kalala, F.
Iliakis, T.
Zareifi, D.-S.
Kodandreopoulou, E.
Vassilakopoulos, T.
Angelopoulou, M.
Siakantaris, M.
Terpos, E.
Variami, E.
Kollia, P.
Vaiopoulos, G.
Pangalis, G.
Viniou, N.-A.
Περιοδικό:
Clinical Lymphoma Myeloma and Leukemia
Λέξεις-κλειδιά:
latent membrane protein 1; messenger RNA; survivin, adult; aged; apoptosis; article; B cell lymphoma; blood sampling; Epstein Barr virus; female; gene; human; leukemic low grade b cell lymphoma; major clinical study; male; middle aged; protein expression; quantitative analysis; reverse transcription polymerase chain reaction; very elderly; virus detection, Apoptosis; Chronic B lymphoproliferative disorders; Viral lymphomagenesis, Aged; Aged, 80 and over; Apoptosis; Epstein-Barr Virus Infections; Female; Herpesvirus 4, Human; Humans; Inhibitor of Apoptosis Proteins; Leukemia, Lymphocytic, Chronic, B-Cell; Lymphoma, B-Cell; Male; Middle Aged; RNA, Messenger; Viral Matrix Proteins
DOI:
10.1016/j.clml.2013.09.002
