Τίτλος:
Novel adenomatous polyposis coli gene promoter is located 40 kb upstream
of the initiating methionine
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The product of the oncosuppressor adenomatous polyposis coli (APC) gene
is involved in cell cycle arrest and apoptosis and its loss of function
is associated with the development of colorectal carcinogenesis. Its
transcriptional regulation seems rather complex and has not been
completely elucidated up to now. In an attempt to identify the
transcription start sites for the mouse Ape gene we have detected a
novel transcript in mouse embryonic stem (ES) cells and colon tissue.
This transcript contains an untranslated exon, whose flanking sequences
exhibited strong promoter activity in transient transfection
experiments. These results suggest that we have identified a novel
promoter for the mouse Ape gene, localized about 40 kb upstream of the
initiating methionine, which drives expression of the unique Ape
transcript type detected in undifferentiated totipotent ES cells.
Transcripts bearing the novel exon combined either with exon 1 or with
exon 2 were detected in all mouse tissues tested. (C) 2004 Elsevier Inc.
All rights reserved.
Συγγραφείς:
Karagianni, N
Ly, MC
Psarras, S
Chlichlia, K and
Schirrmacher, V
Gounari, F
Khazaie, K
Περιοδικό:
LIFESTYLE GENOMICS
Εκδότης:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Λέξεις-κλειδιά:
adenomatous polyposis coli; promoter; untranslated exon; totipotent stem
cells; 5 ‘-RACE
DOI:
10.1016/j.ygeno.2004.09.005
