Τίτλος:
Evaluation of touch-down real-time PCR based on SYBR Green I fluorescent
dye for the detection of Neisseria meningitidis in clinical samples
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Antibiotic treatment prior to transport or admission of patients to
hospital has reduced the proportion of patients with invasive
meningococcal disease (IMD) from whom Neisseria meningitidis can be
isolated by standard microbiological techniques. Assays to detect the
crgA gene were used to detect meningococcal DNA by both conventional
polymerase chain reaction (PCR) and real-time PCR (RTPCR) in relation to
microbiological diagnosis of cases over two years between 2002 and 2003.
The sensitivity of both PCR assays for culture-confirmed cases was 93%
and the specificity was 98.6%. Agreement between the two PCR assays was
96.2%. The inter- and intra-assay variations and effects of different
amounts of DNA on the melting temperatures were examined. The touchdown
RTPCR based on SYBR Green I fluorescent dye detected and characterized
N. meningitidis in clinical samples within one hour. (C) 2004 Published
by Elsevier B.V. on behalf of the Federation of European Microbiological
Societies.
Συγγραφείς:
Kesanopoulos, K
Tzanakaki, G
Levidiotou, S
Blackwell, C and
Kremastinou, J
Περιοδικό:
FEMS Immunology and Medical Microbiology
Λέξεις-κλειδιά:
Neisseria meningitidis; PCR; real-time PCR; SYBR Green I fluorescent dye
DOI:
10.1016/j.femsim.2004.10.011
