Τίτλος:
Circulating oxidized low density lipoprotein, autoantibodies against
them and homocysteine serum levels in diagnosis and estimation of
severity of coronary artery disease
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The oxidative hypothesis of atherosclerosis proposes that oxidative
modification of low density lipoprotein (LDL) plays a critical role in
atherogenesis. The evaluation of LDL oxidation in vivo is therefore very
important. However, data concerning the evaluation of the above
biochemical marker is very limited in clinical practice. Ibis study was
conducted to test the hypothesis that plasma levels of ox-LDL reflect
atherosclerosis and determine the clinical significance in the
measurement of circulating ox-LDL and autoantibodies against them as
well as their correlation with homocysteine and lipid parameters in the
diagnosis and severity of coronary heart disease. A total of 273 in
dividuals were examined: 41 suffering from unstable angina pectoris
(UAP), 62 from stable angina pectoris (SAP) and 170 healthy control
subjects. We used a sensitive method for detecting ox-LDL that is based
on a direct sandwich technique (ELISA) in which two monoclonal
antibodies are directed against separate antigenic determinants on the
oxidized apolipoprotein-B molecule along with another enzyme immunoassay
designed to determine human antibodies to oxidized LDL (anti-oxLDL)
directly in serum. Total homocysteine (HCY) was evaluated by means of a
fully automated fluorescence polarization immunoassay. Patients with UAP
exhibited marked elevations in oxLDL levels as compared to patients with
SAP (161.2 +/- 28.4 vs. 119.2 +/- 26.6, p < 0.001) and the control
subjects (67 +/- 18.8, p < 0.001). The difference in oxLDL levels
between patients with SAP and the control group was also statistically
significant. Similarly, patients with UAP showed marked elevations in
anti-oxLDL antibodies compared to both patients with SAP (602,2 +/- 62.2
vs. 510.8 +/- 50.3, p < 0.001) and control subjects (368 +/- 79.6, p <
0.001). The difference in anti-oxLDL levels between patients with SAP
and the controls was also statistically significant. OxLDL levels were
not correlated with age in any of the groups studied. Triglycerides,
LDL-cholesterol and total cholesterol were elevated in patients with UAP
as opposed to patients with SAP and the control subjects, while HDL
levels were elevated in the control subjects when compared to patients
with SAP and UAP. Homocysteine levels were elevated in patients
suffering from UAP and SAP when compared to healthy subjects. Patients
with UAP or SAP did not differ on homocysteine levels. Our findings
demonstrate the presence of oxLDL in vivo, its strong association with
coronary artery disease as well as with the severity of the clinical
presentation.
Συγγραφείς:
Faviou, E
Vourli, G
Nounopoulos, C
Zachari, A and
Dionyssiou-Asteriou, A
Περιοδικό:
Free Radical Research
Εκδότης:
TAYLOR & FRANCIS LTD LONDON
Λέξεις-κλειδιά:
atherosclerosis; angina pectoris; LDL oxidation; homocysteine
DOI:
10.1080/10715760500072156
