Τίτλος:
Glucocorticoid receptor isoforms in human hepatocarcinoma HepG2 and SaOS-2 osteosarcoma cells: Presence of glucocorticoid receptor alpha in mitochondria and of glucocorticoid receptor beta in nucleoli
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
In the context of a possible direct action of glucocorticosteroids on mitochondrial transcription and/or apoptosis by way of cognate mitochondrial receptors, the possible localization of glucocorticoid receptors α and β (GRα and GRβ) in mitochondria was explored in human hepatocarcinoma HepG2 and osteosarcoma SaOS-2 cells, in which glucocorticoids exert an anabolic and apoptotic effect, respectively. In both cell types, GRα was detected in mitochondria, in nuclei and in cytosol by immunofluorescence labeling and confocal scanning microscopy, by immunogold electron microscopy and by Western blotting. GRβ was shown to be almost exclusively restricted to the nucleus of the two cell types, being particularly concentrated in nucleoli, pointing to a solely nuclear role of this receptor isoform and to a possible function in nucleoli related processes. Computer analysis identified a putative internal mitochondrial targeting sequence within the glucocorticoid receptor. The demonstration of mitochondrially localized GRα in HepG2 and SaOS-2 cells corroborates previous findings in other cell types and further supports a direct role of this receptor in mitochondrial functions. © 2005 Elsevier Ltd. All rights reserved.
Συγγραφείς:
Psarra, A.-M.G.
Solakidi, S.
Trougakos, I.P.
Margaritis, L.H.
Spyrou, G.
Sekeris, C.E.
Περιοδικό:
International Journal of Biochemistry and Cell Biology
Λέξεις-κλειδιά:
glucocorticoid receptor; glucocorticoid receptor alpha; glucocorticoid receptor beta; unclassified drug, apoptosis; article; cancer cell culture; cell strain HepG2; cell type; computer analysis; confocal laser microscopy; controlled study; cytosol; human; human cell; immunofluorescence; immunogold electron microscopy; liver cell carcinoma; mitochondrion; nucleolus; osteosarcoma cell; Western blotting, Blotting, Western; Carcinoma, Hepatocellular; Cell Fractionation; Cell Nucleolus; Humans; Immunohistochemistry; Microscopy, Confocal; Microscopy, Electron; Mitochondria; Osteosarcoma; Receptors, Glucocorticoid; Tumor Cells, Cultured
DOI:
10.1016/j.biocel.2005.06.015
