Περίληψη:
Background. Telomerase preserves telomere length and structure,
preventing cellular senescence, which is associated with alteration of
the chromosomal ends. We hypothesized that telomerase activity is
altered in peripheral blood mononuclear cells (PBMCs) of hemodialysis
(HD) patients. To investigate this hypothesis as well as the
relationship between telomerase and inflammation, we measured the
activity of this reverse transcriptase as well as the level of several
inflammatory markers in PBMCs and serum of an end-stage renal failure
(ESRF) population and a non-renal-failure group of subjects. Methods: In
PBMCs isolated from 42 HD and 39 non-renal-failure subjects of the same
age (51.0 +/- 12.4 and 51.4 +/- 12.1 years, respectively) telomerase
activity was measured using PCR-ELISA; the method was based on the
telomeric repeat amplification protocol. Results: Telomerase activity in
PBMCs was detected in 18 (42.9%) HD and 28 (71.8%) non-renal-failure
subjects (p=0.013). Among positive subjects, percent telomerase activity
in PBMCs was significantly higher in non-renal-failure (117 +/- 112%)
than in HD (47.6 +/- 57.1%) subjects (p=0.008). Detectable telomerase
activity was lower in long-term than in short-term HD patients (13.3 +/-
8.9 vs. 75.0 +/- 64.8%, respectively, p=0.015). Although higher in HD
group, inflammatory indexes (C-reactive protein, interleukin-6, IL-6,
soluble IL-6 and soluble gp130) were not correlated to telomerase
activity in PBMCs. Conclusion:Telomerase activity in PBMCs is reduced in
HD patients. It seems that, at least in this type of cell in this
population, defense from senescence, as assessed by telomerase activity,
is altered and associated with the chronicity of uremia/HD procedure.
Copyright (C) 2006 S. Karger AG, Basel.
Συγγραφείς:
Tsirpanlis, G
Chatzipanagiotou, S
Boufidou, F
Kordinas, V
and Alevyzaki, F
Zoga, M
Kyritsis, I
Stamatelou, K and
Triantafyllis, G
Nicolaou, C
