Περίληψη:
The role of blood monocytes in the secretion of soluble triggering
receptor expressed on myeloiod cells (sTREM-1) was studied in 90
patients with septic syndrome due to ventilator-associated pneumonia.
Blood monocytes were isolated on 7 consecutive d after initiation of
symptoms. Monocytes were incubated in the absence or presence of LPS and
concentrations of sTREM-1 and TNF alpha in cell supernatants and serum
were estimated by an enzyme-immunoassay. sTREM-1 and TNF alpha were
consistently present at detectable levels in the cell supernatants. LPS
induced increased levels of TNFa but not of sTREM-1. Supernatants
recovered from monocytes on d 1 showed levels of sTREM-1 higher than
those recovered on any of the following 6 d (p < 0.05); these levels
were higher in non-survivors than in survivors. Supernatants recovered
from monocytes on d 1 of patients with severe sepsis had elevated
concentrations of sTREM-1 compared to patients with septic shock and
similar to patients with sepsis. A negative correlation was found
between levels of sTREM-1 in the cell supernatants and the percentage of
apoptotic monocytes. In essence, the above results suggest that
monocytes contribute to the production of sTREM-1 in the event of septic
syndrome.
Συγγραφείς:
Plachouras, Diamantis
Routsi, Christina
Giamarellos-Bourboulis,
Evangelos J.
Spyridaki, Ekaterini
Andrianakis, Ilias and
Metzelopoulos, Spyridon
Tsaganos, Thomas
Floros, Ioannis and
Douzinas, Emmanuel E.
Armaganidis, Apostolos
Roussos, Charis and
Giamarellou, Helen
