Τίτλος:
Expression of the O-linked N-acetylglucosamine containing epitope H in
normal myometrium and uterine smooth muscle cell tumors
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Epitope H contains an O-linked N-acetylglucosamine (O-GlcNAc) residue in
a specific conformation and/or environment recognized by monoclonal
antibody H (mAbH). We have previously shown that epitope H is present in
more than one polypeptide and in various types of normal and
pathological cells. In the present study, we focused on uterine smooth
muscle cell tumors and their adjacent normal myometrium to gain further
insight into the expression patterns of epitope H in human tissues.
The indirect immunoperoxidase method was applied using the mAbH and the
monoclonal anti-cytokeratin 8 antibody (AbCK8) in 50 cases of typical
uterine leiomyomas and in five cases of uterine leiomyosarcomas, with
four cases belonging to Group II A and one to Group III according to
Bell et at. [6]. Western immunoblotting was applied using mAbH and
AbCK8 in five cases of uterine leiomyomas and their adjacent myometrium.
The main results were as follows: (1) epitope H showed intense
immunohistochemical expression in 46% (23/50) and moderate expression
in 54% (27/50) of uterine leiomyomas, (2) epitope H showed intense
immunohistochemical expression in 40% (2/5) and moderate expression in
60% (3/5) of uterine leiomyosarcomas, (3) epitope H showed no
difference in the immunohistochemical expression between leiomyomas and
their adjacent myometrium and between leiomyosarcomas and their adjacent
myometrium, (4) immunohistochernical expression of cytokeratin 8 was not
detected in the normal and neoplastic smooth muscle cells, (5) Western
immunoblotting showed that in the smooth muscle cells of the myometrium
and leiomyomas, epitope H is localized in four polypeptides with
molecular weights of 100, 61, 59, and 54 kDa, and (6) Western
immunoblotting did not detect cytokeratin 8 in the normal and neoplastic
smooth muscle cells.
The present results indicate fluctuations of the epitope expression
levels in uterine smooth muscle cell tumors and their adjacent
myometrium. These fluctuations may be of interest for gaining insight
into the pathogenesis of uterine smooth muscle cell tumors, since
O-GlcNAc glycosylation is involved in cell cycle and apoptosis pathways
and may modify proteins involved in oncogenesis (tumor suppressor
proteins and oncoproteins) and proteins with important biological
functions such as cytoskeletal proteins, transcription factors, and
heat-shock proteins. Furthermore, the present results indicate that
cytokeratin 8, without being present in the cells of the myometrium,
leiomyomas and leiomyosarcomas, shares its epitope H, which contains its
unique sugar O-N-acetylglucosamine residue, with four other unrelated
polypeptides produced by the normal and neoplastic smooth muscle cells.
This should be considered when using anti-cytokeratin 8 antibodies in
immunohistochemistry against smooth muscle cell tumors to avoid false
positive immunohistochemical results. (c) 2006 Elsevier GmbH. All rights
reserved.
Συγγραφείς:
Sgantzos, Markos N.
Galani, Vassiliki
Arvanitis, Leonidas D. and
Charchanti, Antonia
Psathas, Panagiotis
Nakoou, Maria and
Havaki, Sophia
Kallioras, Vassillos
Marinos, Evangelos and
Vamvakopoulos, Nicholas C.
Kittas, Christos
Περιοδικό:
PATHOLOGY RESEARCH AND PRACTICE
Εκδότης:
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Λέξεις-κλειδιά:
epitope H; O-linked N-acetylglucosamine; uterine smooth muscle cell
tumors
DOI:
10.1016/j.prp.2006.08.009
