Τίτλος:
Deciphering anti-MOG IgG antibodies: Clinical and radiological spectrum, and comparison of antibody detection assays
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
IgG antibodies to myelin oligodendrocyte glycoprotein (MOG) detected by cell based assays (CBA) have been identified in a constantly expanding spectrum of CNS demyelinating disorders. However, a universally accepted CBA has not been adopted yet. We aimed to analyze the clinical and radiological features of patients with anti-MOG IgG1-antibodies detected with a live-cell CBA and to compare the three most popular MOG-CBAs. We screened sera from 1300 Greek patients (including 426 patients referred by our 8 clinics) suspected for anti-MOG syndrome, and 120 controls with the live-cell MOG-CBA for IgG1-antibodies. 41 patients, versus 0 controls were seropositive. Clinical, serological and radiological data were available and analyzed for the 21 seropositive patients out of the 426 patients of our clinics. Their phenotypes were: 8 optic neuritis, 3 myelitis, 3 neuromyelitis optica, 2 encephalomyelitis, 2 autoimmune encephalitis and 3 atypical MS. We then retested all sera of our 426 patients with the other two most popular MOG-CBAs for total IgG (a live-cell and a commercial fixed-cell CBAs). Seven IgG1-seropositive patients were seronegative for one or both IgG-CBAs. Yet, all 21 patients had clinical and radiological findings previously described in MOG-antibody associated demyelination disease supporting the high specificity of the IgG1-CBA. In addition, all IgG1-CBA-negative sera were also negative by the IgG-CBAs. Also, all controls were negative by all three assays, except one serum found positive by the live IgG-CBA. Overall, our findings support the wide spectrum of anti-MOG associated demyelinating disorders and the superiority of the MOG-IgG1 CBA over other MOG-CBAs. © 2020 Elsevier B.V.
Συγγραφείς:
Tzartos, J.S.
Karagiorgou, K.
Tzanetakos, D.
Breza, M.
Evangelopoulos, M.E.
Pelidou, S.-H.
Bakirtzis, C.
Nikolaidis, I.
Koutsis, G.
Notas, K.
Chroni, E.
Markakis, I.
Grigoriadis, N.C.
Anagnostouli, M.
Orologas, A.
Parisis, D.
Karapanayiotides, T.
Papadimitriou, D.
Kostadima, V.
Elloul, J.
Xidakis, I.
Maris, T.
Zisimopoulou, P.
Tzartos, S.
Kilidireas, C.
Περιοδικό:
Journal of the Neurological Sciences
Λέξεις-κλειδιά:
azathioprine; beta interferon; corticosteroid; glatiramer; immunoglobulin G antibody; methylprednisolone; mycophenolate mofetil; myelin oligodendrocyte glycoprotein; oligoclonal band; prednisolone; rituximab; autoantibody; immunoglobulin G; myelin oligodendrocyte glycoprotein, acute disseminated encephalomyelitis; adult; allergic encephalitis; antibody blood level; antibody detection; Article; clinical article; controlled study; demyelinating disease; encephalomyelitis; female; Greece; HEK293 cell line; human; live cell imaging; male; multiple sclerosis; myelitis; neuroimaging; nuclear magnetic resonance imaging; optic neuritis; phenotype; pleocytosis; priority journal; diagnostic imaging; myelooptic neuropathy; optic neuritis, Autoantibodies; Humans; Immunoglobulin G; Myelin-Oligodendrocyte Glycoprotein; Neuromyelitis Optica; Optic Neuritis
DOI:
10.1016/j.jns.2020.116673
