Τίτλος:
Expression of the growth factor pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta in the serum, cartilage and subchondral bone of patients with osteoarthritis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objectives: Pleiotrophin is a heparin-binding growth factor expressed in embryonic but not mature cartilage, suggesting a role in cartilage development. Elucidation of the molecular changes observed during the remodelling process in osteoarthritis is of paramount importance. This study aimed to investigate serum pleiotrophin levels and expression of pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta in the cartilage and subchondral bone of osteoarthritis patients. Methods: Serum samples derived from 16osteoarthritis patients and 18 healthy donors. Pleiotrophin and receptor protein tyrosine phosphatase beta/zeta in the cartilage and subchondral bone were studied in 29patients who had undergone total knee or hip replacement for primary osteoarthritis and in 10 control patients without macroscopic osteoarthritis changes. Results: Serum pleiotrophin levels and expression of pleiotrophin in chondrocytes and subchondral bone osteocytes significantly increased in osteoarthritis patients graded Ahlback II to III. Receptor protein tyrosine phosphatase beta/zeta was mainly detected in the subchondral bone osteocytes of patients with moderate osteoarthritis and as disease severity increased, in the osteocytes and bone lining cells of the distant trabeculae. Conclusions: These data render pleiotrophin and receptor protein tyrosine phosphatase beta/zeta promising candidates for further studies towards developing targeted therapeutic schemes for osteoarthritis. © 2012 Société française de rhumatologie.
Συγγραφείς:
Kaspiris, A.
Mikelis, C.
Heroult, M.
Khaldi, L.
Grivas, T.B.
Kouvaras, I.
Dangas, S.
Vasiliadis, E.
Lioté, F.
Courty, J.
Papadimitriou, E.
Περιοδικό:
Joint Bone Spine
Λέξεις-κλειδιά:
pleiotrophin; protein tyrosine phosphatase; protein tyrosine phosphatase beta; protein tyrosine phosphatase zeta; unclassified drug, adult; aged; article; cartilage; cartilage cell; cell migration; clinical article; controlled study; disease severity; female; histology; human; human tissue; immunohistochemistry; immunoprecipitation; male; osteoarthritis; osteocyte; osteophyte; protein expression; synovial fluid; total hip prosthesis; total knee replacement; Western blotting
DOI:
10.1016/j.jbspin.2012.10.024
