Τίτλος:
Plasma biomarkers for the identification of women at risk for
early-onset preeclampsia
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: To identify potential biomarkers in the 1st trimester of
pregnancy for the identification of women destined to develop early
onset preeclampsia (EOPE).Methods: Blood samples were obtained from
pregnant women at 11-13weeks of gestation. Women were followed up until
delivery. Five samples from EOPE complicated pregnancies and 5 from
unaffected ones were analysed using 2-DE and MALDI-TOF-TOF MS/MS. The
altered expression of selected proteins was verified by ELISA in an
extended sample cohort.Results: Twelve proteins were differentially
expressed in the plasma of women who subsequently developed EOPE as
compared to controls. Alpha-1-antitrypsin (A1AT), CD5 antigen-like
molecule (CD5L) Keratin, type I cytoskeletal 9 (K1C9), Myeloid cell
nuclear differentiation antigen (MNDA), Transferrin (TRFE) and Vitamin
D-binding protein (VTDB) were up-regulated with fold changes 3.14, 2.18,
1.53, 1.53, 4.26 3.38 respectively, whereas Alpha-2-HS-glycoprotein
(FETUA), Beta-2-glycoprotein 1 (APOH), Complement factor B (CFAB),
Haptoglobin (HPT), Vitronectin (VTNC) and Zinc-alpha-2-glycoprotein
(ZA2G) were down-regulated with fold changes -0.38, -0.76, -0.24, -0.47,
-0.23, and -0.50 respectively. The down-regulation of APOH, VTNC and HPT
was verified using ELISA.Conclusions: The differentially expressed
proteins represent potential biomarkers for the early screening for
EOPE. Follow-up experiments however are necessary for evaluation.
Συγγραφείς:
Kolialexi, Aggeliki
Tsangaris, George Th
Sifakis, Stavros and
Gourgiotis, Dimitris
Katsafadou, Aggeliki
Lykoudi, Alexandra and
Marmarinos, Antonios
Mavreli, Danai
Pergialiotis, Vassilis and
Fexi, Dimitra
Mavrou, Ariadni
Papaioanou, George K. and
Papantoniou, Nikolas
Περιοδικό:
Expert Review of Proteomics
Εκδότης:
TAYLOR & FRANCIS LTD LONDON
Λέξεις-κλειδιά:
Preeclampsia; early onset preeclampsia; proteomics; 2-DE; mass
spectrometry; biomarkers
DOI:
10.1080/14789450.2017.1291345
